![](/images/graphics-bg.png)
Conditioned Medium Reconditions Hippocampal Neurons against Kainic Acid Induced Excitotoxicity: An In Vitro Study
المؤلفون المشاركون
Bhonde, Ramesh R.
Talakad, Sathyaprabha N.
Bevinahal, Pradeep Kumar K.
Venugopal, Chaitra
Yencharla, Harish Chandra Prasad S.
Chandanala, Shashank
Trichur, Raju R.
Dhanushkodi, Anandh
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-11-20
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Stem cell therapy is gaining attention as a promising treatment option for neurodegenerative diseases.
The functional efficacy of grafted cells is a matter of debate and the recent consensus is that the cellular and functional recoveries might be due to “by-stander” effects of grafted cells.
In the present study, we investigated the neuroprotective effect of conditioned medium (CM) derived from human embryonic kidney (HEK) cells in a kainic acid (KA) induced hippocampal degeneration model system in in vitro condition.
Hippocampal cell line was exposed to KA (200 µM) for 24 hrs (lesion group) whereas, in the treatment group, hippocampal cell line was exposed to KA in combination with HEK-CM (KA + HEK-CM).
We observed that KA exposure to cells resulted in significant neuronal loss.
Interestingly, HEK-CM cotreatment completely attenuated the excitotoxic effects of KA.
In HEK-CM cotreatment group, the cell viability was ~85–95% as opposed to 47% in KA alone group.
Further investigation demonstrated that treatment with HEK-CM stimulated the endogenous cell survival factors like brain derived neurotrophic factors (BDNF) and antiapoptotic factor Bcl-2, revealing the possible mechanism of neuroprotection.
Our results suggest that HEK-CM protects hippocampal neurons against excitotoxicity by stimulating the host’s endogenous cell survival mechanisms.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Bevinahal, Pradeep Kumar K.& Venugopal, Chaitra& Yencharla, Harish Chandra Prasad S.& Chandanala, Shashank& Trichur, Raju R.& Talakad, Sathyaprabha N.…[et al.]. 2014. Conditioned Medium Reconditions Hippocampal Neurons against Kainic Acid Induced Excitotoxicity: An In Vitro Study. Journal of Toxicology،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1043177
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Bevinahal, Pradeep Kumar K.…[et al.]. Conditioned Medium Reconditions Hippocampal Neurons against Kainic Acid Induced Excitotoxicity: An In Vitro Study. Journal of Toxicology No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1043177
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Bevinahal, Pradeep Kumar K.& Venugopal, Chaitra& Yencharla, Harish Chandra Prasad S.& Chandanala, Shashank& Trichur, Raju R.& Talakad, Sathyaprabha N.…[et al.]. Conditioned Medium Reconditions Hippocampal Neurons against Kainic Acid Induced Excitotoxicity: An In Vitro Study. Journal of Toxicology. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1043177
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1043177
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)