Conditioned Medium Reconditions Hippocampal Neurons against Kainic Acid Induced Excitotoxicity: An In Vitro Study
Joint Authors
Bhonde, Ramesh R.
Talakad, Sathyaprabha N.
Bevinahal, Pradeep Kumar K.
Venugopal, Chaitra
Yencharla, Harish Chandra Prasad S.
Chandanala, Shashank
Trichur, Raju R.
Dhanushkodi, Anandh
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-11-20
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Stem cell therapy is gaining attention as a promising treatment option for neurodegenerative diseases.
The functional efficacy of grafted cells is a matter of debate and the recent consensus is that the cellular and functional recoveries might be due to “by-stander” effects of grafted cells.
In the present study, we investigated the neuroprotective effect of conditioned medium (CM) derived from human embryonic kidney (HEK) cells in a kainic acid (KA) induced hippocampal degeneration model system in in vitro condition.
Hippocampal cell line was exposed to KA (200 µM) for 24 hrs (lesion group) whereas, in the treatment group, hippocampal cell line was exposed to KA in combination with HEK-CM (KA + HEK-CM).
We observed that KA exposure to cells resulted in significant neuronal loss.
Interestingly, HEK-CM cotreatment completely attenuated the excitotoxic effects of KA.
In HEK-CM cotreatment group, the cell viability was ~85–95% as opposed to 47% in KA alone group.
Further investigation demonstrated that treatment with HEK-CM stimulated the endogenous cell survival factors like brain derived neurotrophic factors (BDNF) and antiapoptotic factor Bcl-2, revealing the possible mechanism of neuroprotection.
Our results suggest that HEK-CM protects hippocampal neurons against excitotoxicity by stimulating the host’s endogenous cell survival mechanisms.
American Psychological Association (APA)
Bevinahal, Pradeep Kumar K.& Venugopal, Chaitra& Yencharla, Harish Chandra Prasad S.& Chandanala, Shashank& Trichur, Raju R.& Talakad, Sathyaprabha N.…[et al.]. 2014. Conditioned Medium Reconditions Hippocampal Neurons against Kainic Acid Induced Excitotoxicity: An In Vitro Study. Journal of Toxicology،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1043177
Modern Language Association (MLA)
Bevinahal, Pradeep Kumar K.…[et al.]. Conditioned Medium Reconditions Hippocampal Neurons against Kainic Acid Induced Excitotoxicity: An In Vitro Study. Journal of Toxicology No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1043177
American Medical Association (AMA)
Bevinahal, Pradeep Kumar K.& Venugopal, Chaitra& Yencharla, Harish Chandra Prasad S.& Chandanala, Shashank& Trichur, Raju R.& Talakad, Sathyaprabha N.…[et al.]. Conditioned Medium Reconditions Hippocampal Neurons against Kainic Acid Induced Excitotoxicity: An In Vitro Study. Journal of Toxicology. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1043177
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043177