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High Content Imaging and Analysis Enable Quantitative In Situ Assessment of CYP3A4 Using Cryopreserved Differentiated HepaRG Cells
المؤلفون المشاركون
Ranade, Aarati R.
Wilson, Melinda S.
McClanahan, Amy M.
Ball, Andrew J.
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-09-07
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
High-throughput imaging-based hepatotoxicity studies capable of analyzing individual cells in situ hold enormous promise for drug safety testing but are frequently limited by a lack of sufficient metabolically competent human cells.
This study examined cryopreserved HepaRG cells, a human liver cell line which differentiates into both hepatocytes and biliary epithelial cells, to determine if these cells may represent a suitable metabolically competent cellular model for novel High Content Analysis (HCA) applications.
Characterization studies showed that these cells retain many features characteristic of primary human hepatocytes and display significant CYP3A4 and CYP1A2 induction, unlike the HepG2 cell line commonly utilized for HCA studies.
Furthermore, this study demonstrates that CYP3A4 induction can be quantified via a simple image analysis-based method, using HepaRG cells as a model system.
Additionally, data demonstrate that the hepatocyte and biliary epithelial subpopulations characteristic of HepaRG cultures can be separated during analysis simply on the basis of nuclear size measurements.
Proof of concept studies with fluorescent cell function reagents indicated that further multiparametric image-based assessment is achievable with HepaRG.
In summary, image-based screening of metabolically competent human hepatocyte models cells such as HepaRG offers novel approaches for hepatotoxicity assessment and improved drug screening tools.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ranade, Aarati R.& Wilson, Melinda S.& McClanahan, Amy M.& Ball, Andrew J.. 2014. High Content Imaging and Analysis Enable Quantitative In Situ Assessment of CYP3A4 Using Cryopreserved Differentiated HepaRG Cells. Journal of Toxicology،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1043183
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ranade, Aarati R.…[et al.]. High Content Imaging and Analysis Enable Quantitative In Situ Assessment of CYP3A4 Using Cryopreserved Differentiated HepaRG Cells. Journal of Toxicology No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1043183
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ranade, Aarati R.& Wilson, Melinda S.& McClanahan, Amy M.& Ball, Andrew J.. High Content Imaging and Analysis Enable Quantitative In Situ Assessment of CYP3A4 Using Cryopreserved Differentiated HepaRG Cells. Journal of Toxicology. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1043183
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1043183
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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