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High Content Imaging and Analysis Enable Quantitative In Situ Assessment of CYP3A4 Using Cryopreserved Differentiated HepaRG Cells
Joint Authors
Ranade, Aarati R.
Wilson, Melinda S.
McClanahan, Amy M.
Ball, Andrew J.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-09-07
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
High-throughput imaging-based hepatotoxicity studies capable of analyzing individual cells in situ hold enormous promise for drug safety testing but are frequently limited by a lack of sufficient metabolically competent human cells.
This study examined cryopreserved HepaRG cells, a human liver cell line which differentiates into both hepatocytes and biliary epithelial cells, to determine if these cells may represent a suitable metabolically competent cellular model for novel High Content Analysis (HCA) applications.
Characterization studies showed that these cells retain many features characteristic of primary human hepatocytes and display significant CYP3A4 and CYP1A2 induction, unlike the HepG2 cell line commonly utilized for HCA studies.
Furthermore, this study demonstrates that CYP3A4 induction can be quantified via a simple image analysis-based method, using HepaRG cells as a model system.
Additionally, data demonstrate that the hepatocyte and biliary epithelial subpopulations characteristic of HepaRG cultures can be separated during analysis simply on the basis of nuclear size measurements.
Proof of concept studies with fluorescent cell function reagents indicated that further multiparametric image-based assessment is achievable with HepaRG.
In summary, image-based screening of metabolically competent human hepatocyte models cells such as HepaRG offers novel approaches for hepatotoxicity assessment and improved drug screening tools.
American Psychological Association (APA)
Ranade, Aarati R.& Wilson, Melinda S.& McClanahan, Amy M.& Ball, Andrew J.. 2014. High Content Imaging and Analysis Enable Quantitative In Situ Assessment of CYP3A4 Using Cryopreserved Differentiated HepaRG Cells. Journal of Toxicology،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1043183
Modern Language Association (MLA)
Ranade, Aarati R.…[et al.]. High Content Imaging and Analysis Enable Quantitative In Situ Assessment of CYP3A4 Using Cryopreserved Differentiated HepaRG Cells. Journal of Toxicology No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1043183
American Medical Association (AMA)
Ranade, Aarati R.& Wilson, Melinda S.& McClanahan, Amy M.& Ball, Andrew J.. High Content Imaging and Analysis Enable Quantitative In Situ Assessment of CYP3A4 Using Cryopreserved Differentiated HepaRG Cells. Journal of Toxicology. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1043183
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043183