Multitracer Stable Isotope Quantification of Arginase and Nitric Oxide Synthase Activity in a Mouse Model of Pseudomonas Lung Infection
المؤلفون المشاركون
Jaecklin, Thomas
Mehl, Anne
Huang, Hailu
Rafii, Mahroukh
Pencharz, Paul
Ratjen, Felix
Grasemann, Hartmut
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-08-11
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Cystic fibrosis airways are deficient for L-arginine, a substrate for nitric oxide synthases (NOSs) and arginases.
The rationale for this study was to quantify NOS and arginase activity in the mouse lung.
Anesthetized unventilated mice received a primed constant stable isotope intravenous infusion containing labeled L-arginine, ornithine, and citrulline.
The isotopic enrichment of each of the infused isotopomers and its product amino acids were measured in plasma and organ homogenates using liquid chromatography-tandem mass spectrometry.
The effect of infection was studied three days after direct tracheal instillation of Pseudomonas-coated agar beads.
In the infusion model, lung infection resulted in a significant (28-fold) increase in NOS activity in lung but not in trachea, kidney, liver, or plasma.
Absolute rates of arginase activity in solid tissues could not be calculated in this model.
In an isolated lung perfusion model used for comparison increased NOS activity in infected lungs was confirmed (28.5-fold) and lung arginase activity was increased 9.7-fold.
The activity of L-arginine metabolizing enzymes can be measured using stable isotope conversion in the mouse.
Accumulation of L-ornithine in the whole mouse model hindered the exact quantification of arginase activity in the lung, a problem that was overcome utilizing an isolated lung perfusion model.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Grasemann, Hartmut& Jaecklin, Thomas& Mehl, Anne& Huang, Hailu& Rafii, Mahroukh& Pencharz, Paul…[et al.]. 2014. Multitracer Stable Isotope Quantification of Arginase and Nitric Oxide Synthase Activity in a Mouse Model of Pseudomonas Lung Infection. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1043489
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Grasemann, Hartmut…[et al.]. Multitracer Stable Isotope Quantification of Arginase and Nitric Oxide Synthase Activity in a Mouse Model of Pseudomonas Lung Infection. Mediators of Inflammation No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1043489
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Grasemann, Hartmut& Jaecklin, Thomas& Mehl, Anne& Huang, Hailu& Rafii, Mahroukh& Pencharz, Paul…[et al.]. Multitracer Stable Isotope Quantification of Arginase and Nitric Oxide Synthase Activity in a Mouse Model of Pseudomonas Lung Infection. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1043489
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1043489
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر