Multitracer Stable Isotope Quantification of Arginase and Nitric Oxide Synthase Activity in a Mouse Model of Pseudomonas Lung Infection
Joint Authors
Jaecklin, Thomas
Mehl, Anne
Huang, Hailu
Rafii, Mahroukh
Pencharz, Paul
Ratjen, Felix
Grasemann, Hartmut
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-11
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Cystic fibrosis airways are deficient for L-arginine, a substrate for nitric oxide synthases (NOSs) and arginases.
The rationale for this study was to quantify NOS and arginase activity in the mouse lung.
Anesthetized unventilated mice received a primed constant stable isotope intravenous infusion containing labeled L-arginine, ornithine, and citrulline.
The isotopic enrichment of each of the infused isotopomers and its product amino acids were measured in plasma and organ homogenates using liquid chromatography-tandem mass spectrometry.
The effect of infection was studied three days after direct tracheal instillation of Pseudomonas-coated agar beads.
In the infusion model, lung infection resulted in a significant (28-fold) increase in NOS activity in lung but not in trachea, kidney, liver, or plasma.
Absolute rates of arginase activity in solid tissues could not be calculated in this model.
In an isolated lung perfusion model used for comparison increased NOS activity in infected lungs was confirmed (28.5-fold) and lung arginase activity was increased 9.7-fold.
The activity of L-arginine metabolizing enzymes can be measured using stable isotope conversion in the mouse.
Accumulation of L-ornithine in the whole mouse model hindered the exact quantification of arginase activity in the lung, a problem that was overcome utilizing an isolated lung perfusion model.
American Psychological Association (APA)
Grasemann, Hartmut& Jaecklin, Thomas& Mehl, Anne& Huang, Hailu& Rafii, Mahroukh& Pencharz, Paul…[et al.]. 2014. Multitracer Stable Isotope Quantification of Arginase and Nitric Oxide Synthase Activity in a Mouse Model of Pseudomonas Lung Infection. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1043489
Modern Language Association (MLA)
Grasemann, Hartmut…[et al.]. Multitracer Stable Isotope Quantification of Arginase and Nitric Oxide Synthase Activity in a Mouse Model of Pseudomonas Lung Infection. Mediators of Inflammation No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1043489
American Medical Association (AMA)
Grasemann, Hartmut& Jaecklin, Thomas& Mehl, Anne& Huang, Hailu& Rafii, Mahroukh& Pencharz, Paul…[et al.]. Multitracer Stable Isotope Quantification of Arginase and Nitric Oxide Synthase Activity in a Mouse Model of Pseudomonas Lung Infection. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1043489
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043489