![](/images/graphics-bg.png)
Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells
المؤلفون المشاركون
Jepsen, Søren
Jäger, Andreas
Deschner, James
Cirelli, Joni Augusto
Bourauel, Christoph
Nokhbehsaim, Marjan
Eick, Sigrun
Nogueira, Andressa Vilas Boas
Rossa, Carlos
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-05-25
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
The present study aimed to evaluate in vitro whether biomechanical loading modulates proinflammatory and bone remodeling mediators production by periodontal ligament (PDL) cells in the presence of bacterial challenge.
Cells were seeded on BioFlex culture plates and exposed to Fusobacterium nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL) and high (CTSH) magnitudes for 1 and 3 days.
Synthesis of cyclooxygenase-2 (COX2) and prostaglandin E2 (PGE2) was evaluated by ELISA.
Gene expression and protein secretion of osteoprotegerin (OPG) and receptor activator of nuclear factor kappa-B ligand (RANKL) were evaluated by quantitative RT-PCR and ELISA, respectively.
F.
nucleatum increased the production of COX2 and PGE2, which was further increased by CTS.
F.
nucleatum-induced increase of PGE2 synthesis was significantly (P<0.05) increased when CTSH was applied at 1 and 3 days.
In addition, CTSH inhibited the F.
nucleatum-induced upregulation of OPG at 1 and 3 days, thereby increasing the RANKL/OPG ratio.
OPG and RANKL mRNA results correlated with the protein results.
In summary, our findings provide original evidence that CTS can enhance bacterial-induced syntheses of molecules associated with inflammation and bone resorption by PDL cells.
Therefore, biomechanical, such as orthodontic or occlusal, loading may enhance the bacterial-induced inflammation and destruction in periodontitis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Nogueira, Andressa Vilas Boas& Nokhbehsaim, Marjan& Eick, Sigrun& Bourauel, Christoph& Jäger, Andreas& Jepsen, Søren…[et al.]. 2014. Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1043555
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Nogueira, Andressa Vilas Boas…[et al.]. Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells. Mediators of Inflammation No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1043555
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Nogueira, Andressa Vilas Boas& Nokhbehsaim, Marjan& Eick, Sigrun& Bourauel, Christoph& Jäger, Andreas& Jepsen, Søren…[et al.]. Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1043555
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1043555
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)