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Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells
Joint Authors
Jepsen, Søren
Jäger, Andreas
Deschner, James
Cirelli, Joni Augusto
Bourauel, Christoph
Nokhbehsaim, Marjan
Eick, Sigrun
Nogueira, Andressa Vilas Boas
Rossa, Carlos
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-25
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The present study aimed to evaluate in vitro whether biomechanical loading modulates proinflammatory and bone remodeling mediators production by periodontal ligament (PDL) cells in the presence of bacterial challenge.
Cells were seeded on BioFlex culture plates and exposed to Fusobacterium nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL) and high (CTSH) magnitudes for 1 and 3 days.
Synthesis of cyclooxygenase-2 (COX2) and prostaglandin E2 (PGE2) was evaluated by ELISA.
Gene expression and protein secretion of osteoprotegerin (OPG) and receptor activator of nuclear factor kappa-B ligand (RANKL) were evaluated by quantitative RT-PCR and ELISA, respectively.
F.
nucleatum increased the production of COX2 and PGE2, which was further increased by CTS.
F.
nucleatum-induced increase of PGE2 synthesis was significantly (P<0.05) increased when CTSH was applied at 1 and 3 days.
In addition, CTSH inhibited the F.
nucleatum-induced upregulation of OPG at 1 and 3 days, thereby increasing the RANKL/OPG ratio.
OPG and RANKL mRNA results correlated with the protein results.
In summary, our findings provide original evidence that CTS can enhance bacterial-induced syntheses of molecules associated with inflammation and bone resorption by PDL cells.
Therefore, biomechanical, such as orthodontic or occlusal, loading may enhance the bacterial-induced inflammation and destruction in periodontitis.
American Psychological Association (APA)
Nogueira, Andressa Vilas Boas& Nokhbehsaim, Marjan& Eick, Sigrun& Bourauel, Christoph& Jäger, Andreas& Jepsen, Søren…[et al.]. 2014. Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1043555
Modern Language Association (MLA)
Nogueira, Andressa Vilas Boas…[et al.]. Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells. Mediators of Inflammation No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1043555
American Medical Association (AMA)
Nogueira, Andressa Vilas Boas& Nokhbehsaim, Marjan& Eick, Sigrun& Bourauel, Christoph& Jäger, Andreas& Jepsen, Søren…[et al.]. Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1043555
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043555