Splenic Stromal Cells from Aged Mice Produce Higher Levels ofIL-6 Compared to Young Mice

الملخص EN

Inflamm-aging indicates the chronic inflammatory state resulting from increased secretion of proinflammatory cytokines and mediators such as IL-6 in the elderly.

Our principle objective was to identify cell types that were affected with aging concerning IL-6 secretion in the murine model.

We compared IL-6 production in spleen cells from both young and aged mice and isolated several types of cells from spleen and investigated IL-6 mRNA expression and protein production.

IL-6 protein productions in cultured stromal cells from aged mice spleen were significantly high compared to young mice upon LPS stimulation.

IL-6 mRNA expression level of freshly isolated stromal cells from aged mice was high compared to young mice.

Furthermore, stromal cells of aged mice highly expressed IL-6 mRNA after LPS injection in vivo.

These results suggest that stromal cells play a role in producing IL-6 in aged mice and imply that they contribute to the chronic inflammatory condition in the elderly.