New Insights into the Steen Solution Properties: Breakthrough in Antioxidant Effects via NOX2 Downregulation

المؤلفون المشاركون

De Falco, Elena
Carnevale, Roberto
Pignatelli, Pasquale
Chimenti, Isotta
Venuta, Federico
Peruzzi, Mariangela
Marullo, Antonino G. M.
Frati, Giacomo
Biondi-Zoccai, Giuseppe
Anile, Marco
Diso, Daniele
Cavarretta, Elena
Sartini, Patrizio
Violi, Francesco

المصدر

Oxidative Medicine and Cellular Longevity

العدد

المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-10، 10ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2014-04-16

دولة النشر

مصر

عدد الصفحات

10

التخصصات الرئيسية

الأحياء

الملخص EN

Ex vivo lung perfusion (EVLP) allows perfusion and reconditioning of retrieved lungs for organ transplantation.

The Steen solution is specifically designed for this procedure but the mechanism through which it elicits its activity is still to be fully clarified.

We speculated that Steen solution may encompass antioxidant properties allowing a reestablishment of pulmonary tissue homeostasis.

Blood samples from 10 healthy volunteers were recruited.

Platelets and white cells were incubated with Steen solution or buffer solution as control and stimulated with suitable agonists.

Reactive oxidant species (ROS), soluble NOX2 (sNOX2-derived peptide), a marker of NADPH oxidase activation, p 47 phox translocation to cell membrane and isoprostanes production, as marker of oxidative stress, and nitric oxide (NO), a powerful vasodilator and antioxidant molecule, were measured upon cell stimulation.

The Steen solution significantly inhibited p 47 phox translocation and NOX2 activation in platelets and white cells.

Consistent with this finding was the reduction of oxidative stress as documented by a significantly lowered formation of ROS and isoprostanes by both platelets and white cells.

Finally, cell incubation with Steen solution resulted in enhanced generation of NO.

Herewith, we provide the first evidence that Steen solution possesses antioxidant properties via downregulation of NADPH oxidase activity and enhanced production of NO.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Carnevale, Roberto& Biondi-Zoccai, Giuseppe& Peruzzi, Mariangela& De Falco, Elena& Chimenti, Isotta& Venuta, Federico…[et al.]. 2014. New Insights into the Steen Solution Properties: Breakthrough in Antioxidant Effects via NOX2 Downregulation. Oxidative Medicine and Cellular Longevity،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1046979

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Carnevale, Roberto…[et al.]. New Insights into the Steen Solution Properties: Breakthrough in Antioxidant Effects via NOX2 Downregulation. Oxidative Medicine and Cellular Longevity No. 2014 (Dec. 2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1046979

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Carnevale, Roberto& Biondi-Zoccai, Giuseppe& Peruzzi, Mariangela& De Falco, Elena& Chimenti, Isotta& Venuta, Federico…[et al.]. New Insights into the Steen Solution Properties: Breakthrough in Antioxidant Effects via NOX2 Downregulation. Oxidative Medicine and Cellular Longevity. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1046979

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1046979