Anandamide Protects HT22 Cells Exposed to Hydrogen Peroxide by Inhibiting CB1 Receptor-Mediated Type 2 NADPH Oxidase
المؤلفون المشاركون
Xiong, Lize
Jia, Ji
Ma, Lei
Wu, Mingchun
Zhang, Lei
Zhang, Xiajing
Zhai, Qian
Jiang, Tao
Wang, Qiang
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-16، 16ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-07-17
دولة النشر
مصر
عدد الصفحات
16
التخصصات الرئيسية
الملخص EN
Background.
Endogenous cannabinoid anandamide (AEA) protects neurons from oxidative injury in rodent models; however the mechanism of AEA-induced neuroprotection remains to be determined.
Activation of neuronal NADPH oxidase 2 (Nox2) contributes to oxidative damage of the brain, and inhibition of Nox2 can attenuate cerebral oxidative stress.
We aimed to determine whether the neuronal Nox2 was involved in protection mediated by AEA.
Methods.
The mouse hippocampal neuron cell line HT22 was exposed to hydrogen peroxide (H2O2) to mimic oxidative injury of neurons.
The protective effect of AEA was assessed by measuring cell metabolic activity, apoptosis, lactate dehydrogenase (LDH) release, cellular morphology, intracellular reactive oxygen species (ROS), and antioxidant and oxidant levels and Nox2 expression.
Results.
HT22 cells exposed to H2O2 demonstrated morphological changes, decreased LDH release, reduced metabolic activity, increased levels of intracellular ROS and oxidized glutathione (GSSG), reduced levels of superoxide dismutase (SOD), and reduced glutathione (GSH) and increased expression of Nox2.
AEA prevented these effects, a property abolished by simultaneous administration of CB1 antagonist AM251 or CB1-siRNA.
Conclusion.
Nox2 inhibition is involved in AEA-induced cytoprotection against oxidative stress through CB1 activation in HT22 cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jia, Ji& Ma, Lei& Wu, Mingchun& Zhang, Lei& Zhang, Xiajing& Zhai, Qian…[et al.]. 2014. Anandamide Protects HT22 Cells Exposed to Hydrogen Peroxide by Inhibiting CB1 Receptor-Mediated Type 2 NADPH Oxidase. Oxidative Medicine and Cellular Longevity،Vol. 2014, no. 2014, pp.1-16.
https://search.emarefa.net/detail/BIM-1047155
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jia, Ji…[et al.]. Anandamide Protects HT22 Cells Exposed to Hydrogen Peroxide by Inhibiting CB1 Receptor-Mediated Type 2 NADPH Oxidase. Oxidative Medicine and Cellular Longevity No. 2014 (Dec. 2014), pp.1-16.
https://search.emarefa.net/detail/BIM-1047155
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jia, Ji& Ma, Lei& Wu, Mingchun& Zhang, Lei& Zhang, Xiajing& Zhai, Qian…[et al.]. Anandamide Protects HT22 Cells Exposed to Hydrogen Peroxide by Inhibiting CB1 Receptor-Mediated Type 2 NADPH Oxidase. Oxidative Medicine and Cellular Longevity. 2014. Vol. 2014, no. 2014, pp.1-16.
https://search.emarefa.net/detail/BIM-1047155
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1047155
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر