Anandamide Protects HT22 Cells Exposed to Hydrogen Peroxide by Inhibiting CB1 Receptor-Mediated Type 2 NADPH Oxidase
Joint Authors
Xiong, Lize
Jia, Ji
Ma, Lei
Wu, Mingchun
Zhang, Lei
Zhang, Xiajing
Zhai, Qian
Jiang, Tao
Wang, Qiang
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-17
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Background.
Endogenous cannabinoid anandamide (AEA) protects neurons from oxidative injury in rodent models; however the mechanism of AEA-induced neuroprotection remains to be determined.
Activation of neuronal NADPH oxidase 2 (Nox2) contributes to oxidative damage of the brain, and inhibition of Nox2 can attenuate cerebral oxidative stress.
We aimed to determine whether the neuronal Nox2 was involved in protection mediated by AEA.
Methods.
The mouse hippocampal neuron cell line HT22 was exposed to hydrogen peroxide (H2O2) to mimic oxidative injury of neurons.
The protective effect of AEA was assessed by measuring cell metabolic activity, apoptosis, lactate dehydrogenase (LDH) release, cellular morphology, intracellular reactive oxygen species (ROS), and antioxidant and oxidant levels and Nox2 expression.
Results.
HT22 cells exposed to H2O2 demonstrated morphological changes, decreased LDH release, reduced metabolic activity, increased levels of intracellular ROS and oxidized glutathione (GSSG), reduced levels of superoxide dismutase (SOD), and reduced glutathione (GSH) and increased expression of Nox2.
AEA prevented these effects, a property abolished by simultaneous administration of CB1 antagonist AM251 or CB1-siRNA.
Conclusion.
Nox2 inhibition is involved in AEA-induced cytoprotection against oxidative stress through CB1 activation in HT22 cells.
American Psychological Association (APA)
Jia, Ji& Ma, Lei& Wu, Mingchun& Zhang, Lei& Zhang, Xiajing& Zhai, Qian…[et al.]. 2014. Anandamide Protects HT22 Cells Exposed to Hydrogen Peroxide by Inhibiting CB1 Receptor-Mediated Type 2 NADPH Oxidase. Oxidative Medicine and Cellular Longevity،Vol. 2014, no. 2014, pp.1-16.
https://search.emarefa.net/detail/BIM-1047155
Modern Language Association (MLA)
Jia, Ji…[et al.]. Anandamide Protects HT22 Cells Exposed to Hydrogen Peroxide by Inhibiting CB1 Receptor-Mediated Type 2 NADPH Oxidase. Oxidative Medicine and Cellular Longevity No. 2014 (Dec. 2014), pp.1-16.
https://search.emarefa.net/detail/BIM-1047155
American Medical Association (AMA)
Jia, Ji& Ma, Lei& Wu, Mingchun& Zhang, Lei& Zhang, Xiajing& Zhai, Qian…[et al.]. Anandamide Protects HT22 Cells Exposed to Hydrogen Peroxide by Inhibiting CB1 Receptor-Mediated Type 2 NADPH Oxidase. Oxidative Medicine and Cellular Longevity. 2014. Vol. 2014, no. 2014, pp.1-16.
https://search.emarefa.net/detail/BIM-1047155
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047155