Urinary Excretion of Neutrophil Gelatinase-Associated Lipocalin in Diabetic Rats
المؤلفون المشاركون
García-Arroyo, Fernando Enrique
Cristóbal-García, Magdalena
Loredo-Mendoza, María Lilia
Sánchez-Lozada, Laura Gabriela
Tapia, Edilia
Arellano, Abraham
Osorio, Horacio
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-08-27
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Recent studies suggest that tubular damage precedes glomerular damage in the progression of diabetic nephropathy.
Therefore, we evaluated oxidative stress and urinary excretion of tubular proteins as markers of tubular dysfunction.
Methods.
Diabetes was induced in rats by streptozotocin administration (50 mg/kg).
Oxidative stress was assessed by measuring the activity of catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD); additionally, expression levels of 3-nitrotyrosine (3-NT), 4-hydroxynonenal (4-HNE), and oxidized protein (OP) were quantified.
Whole glomerular filtration rate (GFR) was measured.
Urinary excretion of neutrophil gelatinase-associated lipocalin (uNGAL), osteopontin (uOPN), and N-acetyl-β-D-glucosaminidase (uNAG) was also determined.
Results.
Diabetic rats showed an increase in uNGAL excretion 7 days following induction of diabetes.
Diuresis, proteinuria, albuminuria, creatinine clearance, and GFR were significantly increased by 30 days after induction.
Furthermore, there was an increase in both CAT and SOD activity, in addition to 3-NT, 4-HNE, and OP expression levels.
However, GPx activity was lower.
Serum levels of NGAL and OPN, as well as excretion levels of uNGAL, uOPN, and uNAG, were increased in diabetics.
Tubular damage was observed by 7 days after diabetes induction and was further aggravated by 30 days after induction.
Conclusion.
The tubular dysfunction evidenced by urinary excretion of NGAL precedes oxidative stress during diabetes.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Arellano, Abraham& García-Arroyo, Fernando Enrique& Cristóbal-García, Magdalena& Loredo-Mendoza, María Lilia& Tapia, Edilia& Sánchez-Lozada, Laura Gabriela…[et al.]. 2014. Urinary Excretion of Neutrophil Gelatinase-Associated Lipocalin in Diabetic Rats. Oxidative Medicine and Cellular Longevity،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1047170
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Arellano, Abraham…[et al.]. Urinary Excretion of Neutrophil Gelatinase-Associated Lipocalin in Diabetic Rats. Oxidative Medicine and Cellular Longevity No. 2014 (Dec. 2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1047170
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Arellano, Abraham& García-Arroyo, Fernando Enrique& Cristóbal-García, Magdalena& Loredo-Mendoza, María Lilia& Tapia, Edilia& Sánchez-Lozada, Laura Gabriela…[et al.]. Urinary Excretion of Neutrophil Gelatinase-Associated Lipocalin in Diabetic Rats. Oxidative Medicine and Cellular Longevity. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1047170
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1047170
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر