Urinary Excretion of Neutrophil Gelatinase-Associated Lipocalin in Diabetic Rats
Joint Authors
García-Arroyo, Fernando Enrique
Cristóbal-García, Magdalena
Loredo-Mendoza, María Lilia
Sánchez-Lozada, Laura Gabriela
Tapia, Edilia
Arellano, Abraham
Osorio, Horacio
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-27
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Recent studies suggest that tubular damage precedes glomerular damage in the progression of diabetic nephropathy.
Therefore, we evaluated oxidative stress and urinary excretion of tubular proteins as markers of tubular dysfunction.
Methods.
Diabetes was induced in rats by streptozotocin administration (50 mg/kg).
Oxidative stress was assessed by measuring the activity of catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD); additionally, expression levels of 3-nitrotyrosine (3-NT), 4-hydroxynonenal (4-HNE), and oxidized protein (OP) were quantified.
Whole glomerular filtration rate (GFR) was measured.
Urinary excretion of neutrophil gelatinase-associated lipocalin (uNGAL), osteopontin (uOPN), and N-acetyl-β-D-glucosaminidase (uNAG) was also determined.
Results.
Diabetic rats showed an increase in uNGAL excretion 7 days following induction of diabetes.
Diuresis, proteinuria, albuminuria, creatinine clearance, and GFR were significantly increased by 30 days after induction.
Furthermore, there was an increase in both CAT and SOD activity, in addition to 3-NT, 4-HNE, and OP expression levels.
However, GPx activity was lower.
Serum levels of NGAL and OPN, as well as excretion levels of uNGAL, uOPN, and uNAG, were increased in diabetics.
Tubular damage was observed by 7 days after diabetes induction and was further aggravated by 30 days after induction.
Conclusion.
The tubular dysfunction evidenced by urinary excretion of NGAL precedes oxidative stress during diabetes.
American Psychological Association (APA)
Arellano, Abraham& García-Arroyo, Fernando Enrique& Cristóbal-García, Magdalena& Loredo-Mendoza, María Lilia& Tapia, Edilia& Sánchez-Lozada, Laura Gabriela…[et al.]. 2014. Urinary Excretion of Neutrophil Gelatinase-Associated Lipocalin in Diabetic Rats. Oxidative Medicine and Cellular Longevity،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1047170
Modern Language Association (MLA)
Arellano, Abraham…[et al.]. Urinary Excretion of Neutrophil Gelatinase-Associated Lipocalin in Diabetic Rats. Oxidative Medicine and Cellular Longevity No. 2014 (Dec. 2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1047170
American Medical Association (AMA)
Arellano, Abraham& García-Arroyo, Fernando Enrique& Cristóbal-García, Magdalena& Loredo-Mendoza, María Lilia& Tapia, Edilia& Sánchez-Lozada, Laura Gabriela…[et al.]. Urinary Excretion of Neutrophil Gelatinase-Associated Lipocalin in Diabetic Rats. Oxidative Medicine and Cellular Longevity. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1047170
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047170