Pharmacokinetic and Biodistribution Assessment of a Near Infrared-Labeled PSMA-Specific Small Molecule in Tumor-Bearing Mice
المؤلفون المشاركون
Kovar, Joy L.
Cheung, Lael L.
Simpson, Melanie A.
Olive, D. Michael
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-04-07
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Prostate cancer is the most frequently diagnosed cancer in men and often requires surgery.
Use of near infrared (NIR) technologies to perform image-guided surgery may improve accurate delineation of tumor margins.
To facilitate preclinical testing of such outcomes, here we developed and characterized a PSMA-targeted small molecule, YC-27.
IRDye 800CW was conjugated to YC-27 or an anti-PSMA antibody used for reference.
Human 22Rv1, PC3M-LN4, and/or LNCaP prostate tumor cells were exposed to the labeled compounds.
In vivo targeting and clearance properties were determined in tumor-bearing mice.
Organs and tumors were excised and imaged to assess probe localization.
YC-27 exhibited a dose dependent increase in signal upon binding.
Binding specificity and internalization were visualized by microscopy.
In vitro and in vivo blocking studies confirmed YC-27 specificity.
In vivo, YC-27 showed good tumor delineation and tissue contrast at doses as low as 0.25 nmole.
YC-27 was cleared via the kidneys but bound the proximal tubules of the renal cortex and epididymis.
Since PSMA is also broadly expressed on the neovasculature of most tumors, we expect YC-27 will have clinical utility for image-guided surgery and tumor resections.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kovar, Joy L.& Cheung, Lael L.& Simpson, Melanie A.& Olive, D. Michael. 2014. Pharmacokinetic and Biodistribution Assessment of a Near Infrared-Labeled PSMA-Specific Small Molecule in Tumor-Bearing Mice. Prostate Cancer،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1047177
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kovar, Joy L.…[et al.]. Pharmacokinetic and Biodistribution Assessment of a Near Infrared-Labeled PSMA-Specific Small Molecule in Tumor-Bearing Mice. Prostate Cancer No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1047177
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kovar, Joy L.& Cheung, Lael L.& Simpson, Melanie A.& Olive, D. Michael. Pharmacokinetic and Biodistribution Assessment of a Near Infrared-Labeled PSMA-Specific Small Molecule in Tumor-Bearing Mice. Prostate Cancer. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1047177
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1047177
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر