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Pharmacokinetic and Biodistribution Assessment of a Near Infrared-Labeled PSMA-Specific Small Molecule in Tumor-Bearing Mice
Joint Authors
Kovar, Joy L.
Cheung, Lael L.
Simpson, Melanie A.
Olive, D. Michael
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-07
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Prostate cancer is the most frequently diagnosed cancer in men and often requires surgery.
Use of near infrared (NIR) technologies to perform image-guided surgery may improve accurate delineation of tumor margins.
To facilitate preclinical testing of such outcomes, here we developed and characterized a PSMA-targeted small molecule, YC-27.
IRDye 800CW was conjugated to YC-27 or an anti-PSMA antibody used for reference.
Human 22Rv1, PC3M-LN4, and/or LNCaP prostate tumor cells were exposed to the labeled compounds.
In vivo targeting and clearance properties were determined in tumor-bearing mice.
Organs and tumors were excised and imaged to assess probe localization.
YC-27 exhibited a dose dependent increase in signal upon binding.
Binding specificity and internalization were visualized by microscopy.
In vitro and in vivo blocking studies confirmed YC-27 specificity.
In vivo, YC-27 showed good tumor delineation and tissue contrast at doses as low as 0.25 nmole.
YC-27 was cleared via the kidneys but bound the proximal tubules of the renal cortex and epididymis.
Since PSMA is also broadly expressed on the neovasculature of most tumors, we expect YC-27 will have clinical utility for image-guided surgery and tumor resections.
American Psychological Association (APA)
Kovar, Joy L.& Cheung, Lael L.& Simpson, Melanie A.& Olive, D. Michael. 2014. Pharmacokinetic and Biodistribution Assessment of a Near Infrared-Labeled PSMA-Specific Small Molecule in Tumor-Bearing Mice. Prostate Cancer،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1047177
Modern Language Association (MLA)
Kovar, Joy L.…[et al.]. Pharmacokinetic and Biodistribution Assessment of a Near Infrared-Labeled PSMA-Specific Small Molecule in Tumor-Bearing Mice. Prostate Cancer No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1047177
American Medical Association (AMA)
Kovar, Joy L.& Cheung, Lael L.& Simpson, Melanie A.& Olive, D. Michael. Pharmacokinetic and Biodistribution Assessment of a Near Infrared-Labeled PSMA-Specific Small Molecule in Tumor-Bearing Mice. Prostate Cancer. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1047177
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047177