Effect of DiD Carbocyanine Dye Labeling on Immunoregulatory Function and Differentiation of Mice Mesenchymal Stem Cells
المؤلفون المشاركون
Mohtasebi, Maryam Sadat
Nasri, Fatemeh
Kamali Sarvestani, Eskandar
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-12-11
دولة النشر
مصر
عدد الصفحات
10
الملخص EN
Mesenchymal stem cells (MSCs) have been used to treat a variety of degenerative disorders.
Labeling of MSCs with an appropriate tracer is vital to demonstrate the in vivo engraftment and differentiation of transplanted MSCs.
DiD is a lipophilic fluorescent dye with near infrared emission spectra that makes it suitable for in vivo tracing.
Therefore, in the present study the consequences of DiD labeling on induction of oxidative stress and apoptosis as well as inhibition of biological functions of mesenchymal stem cells (MSCs) were investigated.
DiD labeling did not provoke the production of ROS, induction of apoptosis, or inhibition of production of immunosuppressive factors (PGE2 and IL-10) by MSCs.
In addition, there were no statistical differences between DiD-labeled and unlabeled MSCs in suppression of proliferation and cytokine production (IFN- γ and IL-17) by in vitro stimulated splenocytes or improvement of clinical score in EAE after in vivo administration.
In addition, DiD labeling did not alter the differentiation capacity of MSCs.
Taken together, DiD can be considered as a safe dye for in vivo tracking of MSCs.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mohtasebi, Maryam Sadat& Nasri, Fatemeh& Kamali Sarvestani, Eskandar. 2014. Effect of DiD Carbocyanine Dye Labeling on Immunoregulatory Function and Differentiation of Mice Mesenchymal Stem Cells. Stem Cells International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1047520
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mohtasebi, Maryam Sadat…[et al.]. Effect of DiD Carbocyanine Dye Labeling on Immunoregulatory Function and Differentiation of Mice Mesenchymal Stem Cells. Stem Cells International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1047520
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mohtasebi, Maryam Sadat& Nasri, Fatemeh& Kamali Sarvestani, Eskandar. Effect of DiD Carbocyanine Dye Labeling on Immunoregulatory Function and Differentiation of Mice Mesenchymal Stem Cells. Stem Cells International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1047520
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1047520
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر