Efficacy of 1.5% Dish Washing Solution and 95% Lemon Water in Substituting Perilous Xylene as a Deparaffinizing Agent for Routine H and E Staining Procedure: A Short Study
المؤلفون المشاركون
Anuradha, A.
Kiran Kumar Naik, B.
Vijay Srinivas, G.
Namala, Srilekha
Ramprasad, V. V. S.
Ramisetty, Sabitha Devi
Udayashankar, Urmila
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-03-31
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Aim.
To assess the efficacy of dish washing solution and diluted lemon water in deparaffinizing sections during conventional hematoxylin and eosin staining technique.
Objective.
The objective is to utilize eco-friendly economical substitute for xylene.
Materials and Methods.
Using twenty paraffin embedded tissue blocks, three sections each were prepared.
One section was stained with conventional H and E method (Group A) and the other two sections with xylene-free (XF) H and E (Groups B and C).
Staining characteristics were compared with xylene and scoring was given.
Total score of 3–5 was regarded as adequate for diagnosis and less than that inadequate for diagnosis.
Statistical Analysis.
Chi-square test, Kruskal Wallis ANOVA test, and Mann-Whitney U test were used.
Results.
Adequacy of nuclear staining, crispness, and staining for diagnosis were greater in both Groups A and C (100%) than Group B (95%).
Adequacy of cytoplasmic staining was similar in all the three groups (100%).
Group B showed comparatively superior uniform staining and less retention of wax.
Conclusion.
Dish washing solution or diluted lemon water can be replaced for xylene as deparaffinizing agent in hematoxylin and eosin procedure.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Anuradha, A.& Namala, Srilekha& Vijay Srinivas, G.& Ramprasad, V. V. S.& Ramisetty, Sabitha Devi& Udayashankar, Urmila…[et al.]. 2014. Efficacy of 1.5% Dish Washing Solution and 95% Lemon Water in Substituting Perilous Xylene as a Deparaffinizing Agent for Routine H and E Staining Procedure: A Short Study. Scientifica،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1047583
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Anuradha, A.…[et al.]. Efficacy of 1.5% Dish Washing Solution and 95% Lemon Water in Substituting Perilous Xylene as a Deparaffinizing Agent for Routine H and E Staining Procedure: A Short Study. Scientifica No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1047583
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Anuradha, A.& Namala, Srilekha& Vijay Srinivas, G.& Ramprasad, V. V. S.& Ramisetty, Sabitha Devi& Udayashankar, Urmila…[et al.]. Efficacy of 1.5% Dish Washing Solution and 95% Lemon Water in Substituting Perilous Xylene as a Deparaffinizing Agent for Routine H and E Staining Procedure: A Short Study. Scientifica. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1047583
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1047583
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر