Efficacy of 1.5% Dish Washing Solution and 95% Lemon Water in Substituting Perilous Xylene as a Deparaffinizing Agent for Routine H and E Staining Procedure: A Short Study
Joint Authors
Anuradha, A.
Kiran Kumar Naik, B.
Vijay Srinivas, G.
Namala, Srilekha
Ramprasad, V. V. S.
Ramisetty, Sabitha Devi
Udayashankar, Urmila
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-31
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Aim.
To assess the efficacy of dish washing solution and diluted lemon water in deparaffinizing sections during conventional hematoxylin and eosin staining technique.
Objective.
The objective is to utilize eco-friendly economical substitute for xylene.
Materials and Methods.
Using twenty paraffin embedded tissue blocks, three sections each were prepared.
One section was stained with conventional H and E method (Group A) and the other two sections with xylene-free (XF) H and E (Groups B and C).
Staining characteristics were compared with xylene and scoring was given.
Total score of 3–5 was regarded as adequate for diagnosis and less than that inadequate for diagnosis.
Statistical Analysis.
Chi-square test, Kruskal Wallis ANOVA test, and Mann-Whitney U test were used.
Results.
Adequacy of nuclear staining, crispness, and staining for diagnosis were greater in both Groups A and C (100%) than Group B (95%).
Adequacy of cytoplasmic staining was similar in all the three groups (100%).
Group B showed comparatively superior uniform staining and less retention of wax.
Conclusion.
Dish washing solution or diluted lemon water can be replaced for xylene as deparaffinizing agent in hematoxylin and eosin procedure.
American Psychological Association (APA)
Anuradha, A.& Namala, Srilekha& Vijay Srinivas, G.& Ramprasad, V. V. S.& Ramisetty, Sabitha Devi& Udayashankar, Urmila…[et al.]. 2014. Efficacy of 1.5% Dish Washing Solution and 95% Lemon Water in Substituting Perilous Xylene as a Deparaffinizing Agent for Routine H and E Staining Procedure: A Short Study. Scientifica،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1047583
Modern Language Association (MLA)
Anuradha, A.…[et al.]. Efficacy of 1.5% Dish Washing Solution and 95% Lemon Water in Substituting Perilous Xylene as a Deparaffinizing Agent for Routine H and E Staining Procedure: A Short Study. Scientifica No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1047583
American Medical Association (AMA)
Anuradha, A.& Namala, Srilekha& Vijay Srinivas, G.& Ramprasad, V. V. S.& Ramisetty, Sabitha Devi& Udayashankar, Urmila…[et al.]. Efficacy of 1.5% Dish Washing Solution and 95% Lemon Water in Substituting Perilous Xylene as a Deparaffinizing Agent for Routine H and E Staining Procedure: A Short Study. Scientifica. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1047583
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047583