Achyranthes aspera Root Extracts Induce Human Colon Cancer Cell (COLO-205)‎ Death by Triggering the Mitochondrial Apoptosis Pathway and S Phase Cell Cycle Arrest

المؤلفون المشاركون

Arora, Shagun
Tandon, Simran

المصدر

The Scientific World Journal

العدد

المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-15، 15ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2014-10-27

دولة النشر

مصر

عدد الصفحات

15

التخصصات الرئيسية

الطب البشري
تكنولوجيا المعلومات وعلم الحاسوب

الملخص EN

Achyranthes aspera (AA) has been used traditionally for the cure of various disorders.

However, the action of root extracts of AA as anticancer agent and its cellular mechanism remain unclear.

The aim was to screen the antitumor effect of ethanolic (EAA) and aqueous (AAA) root extracts on the growth of colon cancer COLO-205 cells by testing their cytotoxicity, followed by their effect on clonogenicity, migration, and induction of apoptosis.

Mechanisms leading to apoptosis and cell cycle arrest were also investigated by expression studies of caspase-9, caspase-3, Bax, Bcl-2, p16, p21, and p27 genes, followed by flow cytometric analysis for cell cycle distribution.

Cytotoxicity screening of AA extracts indicated greater cytotoxic activity of AAA extract against COLO-205 cells.

A series of events marked by apoptosis revealed loss of cell viability, chromatin condensation, and DNA fragmentation in AAA treated cells to a greater extent.

The mRNA expression levels of caspase-9, caspase-3, Bax, p16, p21, and p27 were markedly increased in the AAA treated cells, along with decreased Bcl-2 expression.

The cell cycle arrest at S phase was detected by flow cytometric analysis after treatment with AAA.

Overall the study signifies the aqueous extracts as a promising therapeutic candidate against cancer.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Arora, Shagun& Tandon, Simran. 2014. Achyranthes aspera Root Extracts Induce Human Colon Cancer Cell (COLO-205) Death by Triggering the Mitochondrial Apoptosis Pathway and S Phase Cell Cycle Arrest. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-1048393

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Arora, Shagun& Tandon, Simran. Achyranthes aspera Root Extracts Induce Human Colon Cancer Cell (COLO-205) Death by Triggering the Mitochondrial Apoptosis Pathway and S Phase Cell Cycle Arrest. The Scientific World Journal No. 2014 (2014), pp.1-15.
https://search.emarefa.net/detail/BIM-1048393

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Arora, Shagun& Tandon, Simran. Achyranthes aspera Root Extracts Induce Human Colon Cancer Cell (COLO-205) Death by Triggering the Mitochondrial Apoptosis Pathway and S Phase Cell Cycle Arrest. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-1048393

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1048393