![](/images/graphics-bg.png)
Achyranthes aspera Root Extracts Induce Human Colon Cancer Cell (COLO-205) Death by Triggering the Mitochondrial Apoptosis Pathway and S Phase Cell Cycle Arrest
Joint Authors
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-10-27
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Achyranthes aspera (AA) has been used traditionally for the cure of various disorders.
However, the action of root extracts of AA as anticancer agent and its cellular mechanism remain unclear.
The aim was to screen the antitumor effect of ethanolic (EAA) and aqueous (AAA) root extracts on the growth of colon cancer COLO-205 cells by testing their cytotoxicity, followed by their effect on clonogenicity, migration, and induction of apoptosis.
Mechanisms leading to apoptosis and cell cycle arrest were also investigated by expression studies of caspase-9, caspase-3, Bax, Bcl-2, p16, p21, and p27 genes, followed by flow cytometric analysis for cell cycle distribution.
Cytotoxicity screening of AA extracts indicated greater cytotoxic activity of AAA extract against COLO-205 cells.
A series of events marked by apoptosis revealed loss of cell viability, chromatin condensation, and DNA fragmentation in AAA treated cells to a greater extent.
The mRNA expression levels of caspase-9, caspase-3, Bax, p16, p21, and p27 were markedly increased in the AAA treated cells, along with decreased Bcl-2 expression.
The cell cycle arrest at S phase was detected by flow cytometric analysis after treatment with AAA.
Overall the study signifies the aqueous extracts as a promising therapeutic candidate against cancer.
American Psychological Association (APA)
Arora, Shagun& Tandon, Simran. 2014. Achyranthes aspera Root Extracts Induce Human Colon Cancer Cell (COLO-205) Death by Triggering the Mitochondrial Apoptosis Pathway and S Phase Cell Cycle Arrest. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-1048393
Modern Language Association (MLA)
Arora, Shagun& Tandon, Simran. Achyranthes aspera Root Extracts Induce Human Colon Cancer Cell (COLO-205) Death by Triggering the Mitochondrial Apoptosis Pathway and S Phase Cell Cycle Arrest. The Scientific World Journal No. 2014 (2014), pp.1-15.
https://search.emarefa.net/detail/BIM-1048393
American Medical Association (AMA)
Arora, Shagun& Tandon, Simran. Achyranthes aspera Root Extracts Induce Human Colon Cancer Cell (COLO-205) Death by Triggering the Mitochondrial Apoptosis Pathway and S Phase Cell Cycle Arrest. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-1048393
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1048393