Continuous Drip Flow System to Develop Biofilm of E. faecalis under Anaerobic Conditions

المؤلفون المشاركون

Cohenca, Nestor
Gonzalez, Ana Maria
Corpus, Erika
Silva-Herzog, Daniel
Aragon-Piña, Antonio
Pozos-Guillen, Amaury

المصدر

The Scientific World Journal

العدد

المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-5، 5ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2014-10-13

دولة النشر

مصر

عدد الصفحات

5

التخصصات الرئيسية

الطب البشري
تكنولوجيا المعلومات وعلم الحاسوب

الملخص EN

Purpose.

To evaluate a structurally mature E.

faecalis biofilm developed under anaerobic/dynamic conditions in an in vitro system.

Methods.

An experimental device was developed using a continuous drip flow system designed to develop biofilm under anaerobic conditions.

The inoculum was replaced every 24 hours with a fresh growth medium for up to 10 days to feed the system.

Gram staining was done every 24 hours to control the microorganism purity.

Biofilms developed under the system were evaluated under the scanning electron microscope (SEM).

Results.

SEM micrographs demonstrated mushroom-shaped structures, corresponding to a mature E.

faecalis biofilm.

In the mature biofilm bacterial cells are totally encased in a polymeric extracellular matrix.

Conclusions.

The proposed in vitro system model provides an additional useful tool to study the biofilm concept in endodontic microbiology, allowing for a better understanding of persistent root canal infections.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Gonzalez, Ana Maria& Corpus, Erika& Pozos-Guillen, Amaury& Silva-Herzog, Daniel& Aragon-Piña, Antonio& Cohenca, Nestor. 2014. Continuous Drip Flow System to Develop Biofilm of E. faecalis under Anaerobic Conditions. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-1050696

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Gonzalez, Ana Maria…[et al.]. Continuous Drip Flow System to Develop Biofilm of E. faecalis under Anaerobic Conditions. The Scientific World Journal No. 2014 (2014), pp.1-5.
https://search.emarefa.net/detail/BIM-1050696

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Gonzalez, Ana Maria& Corpus, Erika& Pozos-Guillen, Amaury& Silva-Herzog, Daniel& Aragon-Piña, Antonio& Cohenca, Nestor. Continuous Drip Flow System to Develop Biofilm of E. faecalis under Anaerobic Conditions. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-1050696

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1050696