Continuous Drip Flow System to Develop Biofilm of E. faecalis under Anaerobic Conditions
Joint Authors
Cohenca, Nestor
Gonzalez, Ana Maria
Corpus, Erika
Silva-Herzog, Daniel
Aragon-Piña, Antonio
Pozos-Guillen, Amaury
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-10-13
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Purpose.
To evaluate a structurally mature E.
faecalis biofilm developed under anaerobic/dynamic conditions in an in vitro system.
Methods.
An experimental device was developed using a continuous drip flow system designed to develop biofilm under anaerobic conditions.
The inoculum was replaced every 24 hours with a fresh growth medium for up to 10 days to feed the system.
Gram staining was done every 24 hours to control the microorganism purity.
Biofilms developed under the system were evaluated under the scanning electron microscope (SEM).
Results.
SEM micrographs demonstrated mushroom-shaped structures, corresponding to a mature E.
faecalis biofilm.
In the mature biofilm bacterial cells are totally encased in a polymeric extracellular matrix.
Conclusions.
The proposed in vitro system model provides an additional useful tool to study the biofilm concept in endodontic microbiology, allowing for a better understanding of persistent root canal infections.
American Psychological Association (APA)
Gonzalez, Ana Maria& Corpus, Erika& Pozos-Guillen, Amaury& Silva-Herzog, Daniel& Aragon-Piña, Antonio& Cohenca, Nestor. 2014. Continuous Drip Flow System to Develop Biofilm of E. faecalis under Anaerobic Conditions. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-1050696
Modern Language Association (MLA)
Gonzalez, Ana Maria…[et al.]. Continuous Drip Flow System to Develop Biofilm of E. faecalis under Anaerobic Conditions. The Scientific World Journal No. 2014 (2014), pp.1-5.
https://search.emarefa.net/detail/BIM-1050696
American Medical Association (AMA)
Gonzalez, Ana Maria& Corpus, Erika& Pozos-Guillen, Amaury& Silva-Herzog, Daniel& Aragon-Piña, Antonio& Cohenca, Nestor. Continuous Drip Flow System to Develop Biofilm of E. faecalis under Anaerobic Conditions. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-1050696
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1050696