Ultrastructure of Plant Leaf Cuticles in relation to Sample Preparation as Observed by Transmission Electron Microscopy
المؤلفون المشاركون
Guzmán, Paula
Fernández, Victoria
Khayet, Mohamed
García, María Luisa
Fernández, Agustín
Gil, Luis
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-04-22
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الطب البشري
تكنولوجيا المعلومات وعلم الحاسوب
الملخص EN
The leaf cuticular ultrastructure of some plant species has been examined by transmission electron microscopy (TEM) in only few studies.
Attending to the different cuticle layers and inner structure, plant cuticles have been grouped into six general morphological types.
With the aim of critically examining the effect of cuticle isolation and preparation for TEM analysis on cuticular ultrastructure, adaxial leaf cuticles of blue-gum eucalypt, grey poplar, and European pear were assessed, following a membrane science approach.
The embedding and staining protocols affected the ultrastructure of the cuticles analysed.
The solubility parameter, surface tension, and contact angles with water of pure Spurr's and LR-White resins were within a similar range.
Differences were however estimated for resin : solvent mixtures, since Spurr’s resin is combined with acetone and LR-White resin is mixed with ethanol.
Given the composite hydrophilic and lipophilic nature of plant cuticles, the particular TEM tissue embedding and staining procedures employed may affect sample ultrastructure and the interpretation of the results in physicochemical and biological terms.
It is concluded that tissue preparation procedures may be optimised to facilitate the observation of the micro- and nanostructure of cuticular layers and components with different degrees of polarity and hydrophobicity.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Guzmán, Paula& Fernández, Victoria& Khayet, Mohamed& García, María Luisa& Fernández, Agustín& Gil, Luis. 2014. Ultrastructure of Plant Leaf Cuticles in relation to Sample Preparation as Observed by Transmission Electron Microscopy. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1051772
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Guzmán, Paula…[et al.]. Ultrastructure of Plant Leaf Cuticles in relation to Sample Preparation as Observed by Transmission Electron Microscopy. The Scientific World Journal No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1051772
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Guzmán, Paula& Fernández, Victoria& Khayet, Mohamed& García, María Luisa& Fernández, Agustín& Gil, Luis. Ultrastructure of Plant Leaf Cuticles in relation to Sample Preparation as Observed by Transmission Electron Microscopy. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1051772
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1051772
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر