Ultrastructure of Plant Leaf Cuticles in relation to Sample Preparation as Observed by Transmission Electron Microscopy
Joint Authors
Guzmán, Paula
Fernández, Victoria
Khayet, Mohamed
García, María Luisa
Fernández, Agustín
Gil, Luis
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-22
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
The leaf cuticular ultrastructure of some plant species has been examined by transmission electron microscopy (TEM) in only few studies.
Attending to the different cuticle layers and inner structure, plant cuticles have been grouped into six general morphological types.
With the aim of critically examining the effect of cuticle isolation and preparation for TEM analysis on cuticular ultrastructure, adaxial leaf cuticles of blue-gum eucalypt, grey poplar, and European pear were assessed, following a membrane science approach.
The embedding and staining protocols affected the ultrastructure of the cuticles analysed.
The solubility parameter, surface tension, and contact angles with water of pure Spurr's and LR-White resins were within a similar range.
Differences were however estimated for resin : solvent mixtures, since Spurr’s resin is combined with acetone and LR-White resin is mixed with ethanol.
Given the composite hydrophilic and lipophilic nature of plant cuticles, the particular TEM tissue embedding and staining procedures employed may affect sample ultrastructure and the interpretation of the results in physicochemical and biological terms.
It is concluded that tissue preparation procedures may be optimised to facilitate the observation of the micro- and nanostructure of cuticular layers and components with different degrees of polarity and hydrophobicity.
American Psychological Association (APA)
Guzmán, Paula& Fernández, Victoria& Khayet, Mohamed& García, María Luisa& Fernández, Agustín& Gil, Luis. 2014. Ultrastructure of Plant Leaf Cuticles in relation to Sample Preparation as Observed by Transmission Electron Microscopy. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1051772
Modern Language Association (MLA)
Guzmán, Paula…[et al.]. Ultrastructure of Plant Leaf Cuticles in relation to Sample Preparation as Observed by Transmission Electron Microscopy. The Scientific World Journal No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1051772
American Medical Association (AMA)
Guzmán, Paula& Fernández, Victoria& Khayet, Mohamed& García, María Luisa& Fernández, Agustín& Gil, Luis. Ultrastructure of Plant Leaf Cuticles in relation to Sample Preparation as Observed by Transmission Electron Microscopy. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1051772
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1051772