Molecular Detection, Phylogenetic Analysis, and Identification of Transcription Motifs in Feline Leukemia Virus from Naturally Infected Cats in Malaysia
المؤلفون المشاركون
Bande, Faruku
Arshad, Siti Suri
Hassan, Latiffah
Zakaria, Zunita
المصدر
Veterinary Medicine International
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-11-17
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
A nested PCR assay was used to determine the viral RNA and proviral DNA status of naturally infected cats.
Selected samples that were FeLV-positive by PCR were subjected to sequencing, phylogenetic analysis, and motifs search.
Of the 39 samples that were positive for FeLV p27 antigen, 87.2% (34/39) were confirmed positive with nested PCR.
FeLV proviral DNA was detected in 38 (97.3%) of p27-antigen negative samples.
Malaysian FeLV isolates are found to be highly similar with a homology of 91% to 100%.
Phylogenetic analysis revealed that Malaysian FeLV isolates divided into two clusters, with a majority (86.2%) sharing similarity with FeLV-K01803 and fewer isolates (13.8%) with FeLV-GM1 strain.
Different enhancer motifs including NF-GMa, Krox-20/WT1I-del2, BAF1, AP-2, TBP, TFIIF-beta, TRF, and TFIID are found to occur either in single, duplicate, triplicate, or sets of 5 in different positions within the U3-LTR-gag region.
The present result confirms the occurrence of FeLV viral RNA and provirus DNA in naturally infected cats.
Malaysian FeLV isolates are highly similar, and a majority of them are closely related to a UK isolate.
This study provides the first molecular based information on FeLV in Malaysia.
Additionally, different enhancer motifs likely associated with FeLV related pathogenesis have been identified.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Bande, Faruku& Arshad, Siti Suri& Hassan, Latiffah& Zakaria, Zunita. 2014. Molecular Detection, Phylogenetic Analysis, and Identification of Transcription Motifs in Feline Leukemia Virus from Naturally Infected Cats in Malaysia. Veterinary Medicine International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1051942
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Bande, Faruku…[et al.]. Molecular Detection, Phylogenetic Analysis, and Identification of Transcription Motifs in Feline Leukemia Virus from Naturally Infected Cats in Malaysia. Veterinary Medicine International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1051942
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Bande, Faruku& Arshad, Siti Suri& Hassan, Latiffah& Zakaria, Zunita. Molecular Detection, Phylogenetic Analysis, and Identification of Transcription Motifs in Feline Leukemia Virus from Naturally Infected Cats in Malaysia. Veterinary Medicine International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1051942
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1051942
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر