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Molecular Detection, Phylogenetic Analysis, and Identification of Transcription Motifs in Feline Leukemia Virus from Naturally Infected Cats in Malaysia
Joint Authors
Bande, Faruku
Arshad, Siti Suri
Hassan, Latiffah
Zakaria, Zunita
Source
Veterinary Medicine International
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-11-17
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
A nested PCR assay was used to determine the viral RNA and proviral DNA status of naturally infected cats.
Selected samples that were FeLV-positive by PCR were subjected to sequencing, phylogenetic analysis, and motifs search.
Of the 39 samples that were positive for FeLV p27 antigen, 87.2% (34/39) were confirmed positive with nested PCR.
FeLV proviral DNA was detected in 38 (97.3%) of p27-antigen negative samples.
Malaysian FeLV isolates are found to be highly similar with a homology of 91% to 100%.
Phylogenetic analysis revealed that Malaysian FeLV isolates divided into two clusters, with a majority (86.2%) sharing similarity with FeLV-K01803 and fewer isolates (13.8%) with FeLV-GM1 strain.
Different enhancer motifs including NF-GMa, Krox-20/WT1I-del2, BAF1, AP-2, TBP, TFIIF-beta, TRF, and TFIID are found to occur either in single, duplicate, triplicate, or sets of 5 in different positions within the U3-LTR-gag region.
The present result confirms the occurrence of FeLV viral RNA and provirus DNA in naturally infected cats.
Malaysian FeLV isolates are highly similar, and a majority of them are closely related to a UK isolate.
This study provides the first molecular based information on FeLV in Malaysia.
Additionally, different enhancer motifs likely associated with FeLV related pathogenesis have been identified.
American Psychological Association (APA)
Bande, Faruku& Arshad, Siti Suri& Hassan, Latiffah& Zakaria, Zunita. 2014. Molecular Detection, Phylogenetic Analysis, and Identification of Transcription Motifs in Feline Leukemia Virus from Naturally Infected Cats in Malaysia. Veterinary Medicine International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1051942
Modern Language Association (MLA)
Bande, Faruku…[et al.]. Molecular Detection, Phylogenetic Analysis, and Identification of Transcription Motifs in Feline Leukemia Virus from Naturally Infected Cats in Malaysia. Veterinary Medicine International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1051942
American Medical Association (AMA)
Bande, Faruku& Arshad, Siti Suri& Hassan, Latiffah& Zakaria, Zunita. Molecular Detection, Phylogenetic Analysis, and Identification of Transcription Motifs in Feline Leukemia Virus from Naturally Infected Cats in Malaysia. Veterinary Medicine International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1051942
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1051942