Identification of Glioblastoma Phosphotyrosine-Containing Proteins with Two-Dimensional Western Blotting and Tandem Mass Spectrometry
المؤلفون المشاركون
Zhan, Xianquan
Guo, Tianyao
Yang, Haiyan
Li, Ling
Peng, Fang
Li, Mao-Yu
Wang, Xiaowei
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-21، 21ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-05-18
دولة النشر
مصر
عدد الصفحات
21
التخصصات الرئيسية
الملخص EN
To investigate the presence of, and the potential biological roles of, protein tyrosine phosphorylation in the glioblastoma pathogenesis, two-dimensional gel electrophoresis- (2DGE-) based Western blotting coupled with liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) analysis was used to detect and identify the phosphotyrosine immunoreaction-positive proteins in a glioblastoma tissue.
MS/MS and Mascot analyses were used to determine the phosphotyrosine sites of each phosphopeptide.
Protein domain and motif analysis and systems pathway analysis were used to determine the protein domains/motifs that contained phosphotyrosine residue and signal pathway networks to clarify the potential biological functions of protein tyrosine phosphorylation.
A total of 24 phosphotyrosine-containing proteins were identified.
Each phosphotyrosine-containing protein contained at least one tyrosine kinase phosphorylation motif and a certain structural and functional domains.
Those phosphotyrosine-containing proteins were involved in the multiple signal pathway systems such as oxidative stress, stress response, and cell migration.
Those data show 2DGE-based Western blotting, MS/MS, and bioinformatics are a set of effective approaches to detect and identify glioblastoma tyrosine-phosphorylated proteome and to effectively rationalize the biological roles of tyrosine phosphorylation in the glioblastoma biological systems.
It provides novel insights regarding tyrosine phosphorylation and its potential role in the molecular mechanism of a glioblastoma.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Guo, Tianyao& Wang, Xiaowei& Li, Mao-Yu& Yang, Haiyan& Li, Ling& Peng, Fang…[et al.]. 2015. Identification of Glioblastoma Phosphotyrosine-Containing Proteins with Two-Dimensional Western Blotting and Tandem Mass Spectrometry. BioMed Research International،Vol. 2015, no. 2015, pp.1-21.
https://search.emarefa.net/detail/BIM-1054306
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Guo, Tianyao…[et al.]. Identification of Glioblastoma Phosphotyrosine-Containing Proteins with Two-Dimensional Western Blotting and Tandem Mass Spectrometry. BioMed Research International No. 2015 (2015), pp.1-21.
https://search.emarefa.net/detail/BIM-1054306
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Guo, Tianyao& Wang, Xiaowei& Li, Mao-Yu& Yang, Haiyan& Li, Ling& Peng, Fang…[et al.]. Identification of Glioblastoma Phosphotyrosine-Containing Proteins with Two-Dimensional Western Blotting and Tandem Mass Spectrometry. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-21.
https://search.emarefa.net/detail/BIM-1054306
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1054306
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر