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Identification of Glioblastoma Phosphotyrosine-Containing Proteins with Two-Dimensional Western Blotting and Tandem Mass Spectrometry
Joint Authors
Zhan, Xianquan
Guo, Tianyao
Yang, Haiyan
Li, Ling
Peng, Fang
Li, Mao-Yu
Wang, Xiaowei
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-21, 21 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-18
Country of Publication
Egypt
No. of Pages
21
Main Subjects
Abstract EN
To investigate the presence of, and the potential biological roles of, protein tyrosine phosphorylation in the glioblastoma pathogenesis, two-dimensional gel electrophoresis- (2DGE-) based Western blotting coupled with liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) analysis was used to detect and identify the phosphotyrosine immunoreaction-positive proteins in a glioblastoma tissue.
MS/MS and Mascot analyses were used to determine the phosphotyrosine sites of each phosphopeptide.
Protein domain and motif analysis and systems pathway analysis were used to determine the protein domains/motifs that contained phosphotyrosine residue and signal pathway networks to clarify the potential biological functions of protein tyrosine phosphorylation.
A total of 24 phosphotyrosine-containing proteins were identified.
Each phosphotyrosine-containing protein contained at least one tyrosine kinase phosphorylation motif and a certain structural and functional domains.
Those phosphotyrosine-containing proteins were involved in the multiple signal pathway systems such as oxidative stress, stress response, and cell migration.
Those data show 2DGE-based Western blotting, MS/MS, and bioinformatics are a set of effective approaches to detect and identify glioblastoma tyrosine-phosphorylated proteome and to effectively rationalize the biological roles of tyrosine phosphorylation in the glioblastoma biological systems.
It provides novel insights regarding tyrosine phosphorylation and its potential role in the molecular mechanism of a glioblastoma.
American Psychological Association (APA)
Guo, Tianyao& Wang, Xiaowei& Li, Mao-Yu& Yang, Haiyan& Li, Ling& Peng, Fang…[et al.]. 2015. Identification of Glioblastoma Phosphotyrosine-Containing Proteins with Two-Dimensional Western Blotting and Tandem Mass Spectrometry. BioMed Research International،Vol. 2015, no. 2015, pp.1-21.
https://search.emarefa.net/detail/BIM-1054306
Modern Language Association (MLA)
Guo, Tianyao…[et al.]. Identification of Glioblastoma Phosphotyrosine-Containing Proteins with Two-Dimensional Western Blotting and Tandem Mass Spectrometry. BioMed Research International No. 2015 (2015), pp.1-21.
https://search.emarefa.net/detail/BIM-1054306
American Medical Association (AMA)
Guo, Tianyao& Wang, Xiaowei& Li, Mao-Yu& Yang, Haiyan& Li, Ling& Peng, Fang…[et al.]. Identification of Glioblastoma Phosphotyrosine-Containing Proteins with Two-Dimensional Western Blotting and Tandem Mass Spectrometry. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-21.
https://search.emarefa.net/detail/BIM-1054306
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054306