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MALDI-TOF Mass Array Analysis of Nell-1 Promoter Methylation Patterns in Human Gastric Cancer
المؤلفون المشاركون
Gao, Changlu
Zhang, Qian
Kong, Deyang
Wu, Di
Su, Changlei
Tong, Jinxue
Chen, Feng
Zhang, Qifan
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-05-19
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Mass spectrometry (MS) enables rapid and sensitive qualitative and quantitative analyses of biomolecules (proteins, peptides, oligosaccharides, lipids, DNA, and RNA), drugs, and metabolites.
MS has become an essential tool in modern biomedical research, including the analysis of DNA methylation.
DNA methylation has been reported in many cancers, suggesting that it can be utilized as an early biomarker to improve the early diagnosis rate.
Using matrix-assisted laser desorption/ionization time-of-flight MS and MassCLEAVE reagent, we compared Nell-1 hypermethylation levels among tumor tissues, paracarcinoma tissues, and normal tissues from gastric cancer patients.
Almost 80% of the CpG sites in the amplicons produced were covered by the analysis.
Our results indicate a significant difference in methylation status between gastric cancer tissue (a higher level) and normal tissue.
The same trend was identified in gastric cancer tissue versus paracarcinoma tissue.
We also detected lower relative expression of Nell-1 by real-time PCR.
Furthermore, immunohistochemical analyses revealed that Nell-1 staining was less intense in cancer tissue relative to normal tissue and that the tumor cells had spread to the muscle layer.
These findings may serve as a guide for the early diagnosis of gastric cancer.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gao, Changlu& Zhang, Qian& Kong, Deyang& Wu, Di& Su, Changlei& Tong, Jinxue…[et al.]. 2015. MALDI-TOF Mass Array Analysis of Nell-1 Promoter Methylation Patterns in Human Gastric Cancer. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1054322
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gao, Changlu…[et al.]. MALDI-TOF Mass Array Analysis of Nell-1 Promoter Methylation Patterns in Human Gastric Cancer. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1054322
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gao, Changlu& Zhang, Qian& Kong, Deyang& Wu, Di& Su, Changlei& Tong, Jinxue…[et al.]. MALDI-TOF Mass Array Analysis of Nell-1 Promoter Methylation Patterns in Human Gastric Cancer. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1054322
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1054322
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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