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MALDI-TOF Mass Array Analysis of Nell-1 Promoter Methylation Patterns in Human Gastric Cancer
Joint Authors
Gao, Changlu
Zhang, Qian
Kong, Deyang
Wu, Di
Su, Changlei
Tong, Jinxue
Chen, Feng
Zhang, Qifan
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-19
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Mass spectrometry (MS) enables rapid and sensitive qualitative and quantitative analyses of biomolecules (proteins, peptides, oligosaccharides, lipids, DNA, and RNA), drugs, and metabolites.
MS has become an essential tool in modern biomedical research, including the analysis of DNA methylation.
DNA methylation has been reported in many cancers, suggesting that it can be utilized as an early biomarker to improve the early diagnosis rate.
Using matrix-assisted laser desorption/ionization time-of-flight MS and MassCLEAVE reagent, we compared Nell-1 hypermethylation levels among tumor tissues, paracarcinoma tissues, and normal tissues from gastric cancer patients.
Almost 80% of the CpG sites in the amplicons produced were covered by the analysis.
Our results indicate a significant difference in methylation status between gastric cancer tissue (a higher level) and normal tissue.
The same trend was identified in gastric cancer tissue versus paracarcinoma tissue.
We also detected lower relative expression of Nell-1 by real-time PCR.
Furthermore, immunohistochemical analyses revealed that Nell-1 staining was less intense in cancer tissue relative to normal tissue and that the tumor cells had spread to the muscle layer.
These findings may serve as a guide for the early diagnosis of gastric cancer.
American Psychological Association (APA)
Gao, Changlu& Zhang, Qian& Kong, Deyang& Wu, Di& Su, Changlei& Tong, Jinxue…[et al.]. 2015. MALDI-TOF Mass Array Analysis of Nell-1 Promoter Methylation Patterns in Human Gastric Cancer. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1054322
Modern Language Association (MLA)
Gao, Changlu…[et al.]. MALDI-TOF Mass Array Analysis of Nell-1 Promoter Methylation Patterns in Human Gastric Cancer. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1054322
American Medical Association (AMA)
Gao, Changlu& Zhang, Qian& Kong, Deyang& Wu, Di& Su, Changlei& Tong, Jinxue…[et al.]. MALDI-TOF Mass Array Analysis of Nell-1 Promoter Methylation Patterns in Human Gastric Cancer. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1054322
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054322