Molecular Epidemiology of Invasive Listeriosis due to Listeria monocytogenes in a Spanish Hospital over a Nine-Year Study Period, 2006–2014
المؤلفون المشاركون
Rodriguez-Lazaro, D.
Ariza-Miguel, Jaime
Fernández-Natal, María Isabel
Soriano, Francisco
Hernández, Marta
Stessl, Beatrix
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-10-11
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
We investigated the pathogenicity, invasiveness, and genetic relatedness of 17 clinical Listeria monocytogenes stains isolated over a period of nine years (2006–2014).
All isolates were phenotypically characterised and growth patterns were determined.
The antimicrobial susceptibility of L.
monocytogenes isolates was determined in E-tests.
Invasion assays were performed with epithelial HeLa cells.
Finally, L.
monocytogenes isolates were subtyped by PFGE and MLST.
All isolates had similar phenotypic characteristics (β-haemolysis and lecithinase activity), and three types of growth curve were observed.
Bacterial recovery rates after invasion assays ranged from 0.09% to 7.26% (1.62 ± 0.46).
MLST identified 11 sequence types (STs), and 14 PFGE profiles were obtained, indicating a high degree of genetic diversity.
Genetic studies unequivocally revealed the occurrence of one outbreak of listeriosis in humans that had not previously been reported.
This outbreak occurred in October 2009 and affected three patients from neighbouring towns.
In conclusion, the molecular epidemiological analysis clearly revealed a cluster (three human cases, all ST1) of not previously reported listeriosis cases in northwestern Spain.
Our findings indicate that molecular subtyping, in combination with epidemiological case analysis, is essential and should be implemented in routine diagnosis, to improve the tracing of the sources of outbreaks.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ariza-Miguel, Jaime& Fernández-Natal, María Isabel& Soriano, Francisco& Hernández, Marta& Stessl, Beatrix& Rodriguez-Lazaro, D.. 2015. Molecular Epidemiology of Invasive Listeriosis due to Listeria monocytogenes in a Spanish Hospital over a Nine-Year Study Period, 2006–2014. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1054564
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ariza-Miguel, Jaime…[et al.]. Molecular Epidemiology of Invasive Listeriosis due to Listeria monocytogenes in a Spanish Hospital over a Nine-Year Study Period, 2006–2014. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1054564
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ariza-Miguel, Jaime& Fernández-Natal, María Isabel& Soriano, Francisco& Hernández, Marta& Stessl, Beatrix& Rodriguez-Lazaro, D.. Molecular Epidemiology of Invasive Listeriosis due to Listeria monocytogenes in a Spanish Hospital over a Nine-Year Study Period, 2006–2014. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1054564
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1054564
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر