Molecular Epidemiology of Invasive Listeriosis due to Listeria monocytogenes in a Spanish Hospital over a Nine-Year Study Period, 2006–2014
Joint Authors
Rodriguez-Lazaro, D.
Ariza-Miguel, Jaime
Fernández-Natal, María Isabel
Soriano, Francisco
Hernández, Marta
Stessl, Beatrix
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-11
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
We investigated the pathogenicity, invasiveness, and genetic relatedness of 17 clinical Listeria monocytogenes stains isolated over a period of nine years (2006–2014).
All isolates were phenotypically characterised and growth patterns were determined.
The antimicrobial susceptibility of L.
monocytogenes isolates was determined in E-tests.
Invasion assays were performed with epithelial HeLa cells.
Finally, L.
monocytogenes isolates were subtyped by PFGE and MLST.
All isolates had similar phenotypic characteristics (β-haemolysis and lecithinase activity), and three types of growth curve were observed.
Bacterial recovery rates after invasion assays ranged from 0.09% to 7.26% (1.62 ± 0.46).
MLST identified 11 sequence types (STs), and 14 PFGE profiles were obtained, indicating a high degree of genetic diversity.
Genetic studies unequivocally revealed the occurrence of one outbreak of listeriosis in humans that had not previously been reported.
This outbreak occurred in October 2009 and affected three patients from neighbouring towns.
In conclusion, the molecular epidemiological analysis clearly revealed a cluster (three human cases, all ST1) of not previously reported listeriosis cases in northwestern Spain.
Our findings indicate that molecular subtyping, in combination with epidemiological case analysis, is essential and should be implemented in routine diagnosis, to improve the tracing of the sources of outbreaks.
American Psychological Association (APA)
Ariza-Miguel, Jaime& Fernández-Natal, María Isabel& Soriano, Francisco& Hernández, Marta& Stessl, Beatrix& Rodriguez-Lazaro, D.. 2015. Molecular Epidemiology of Invasive Listeriosis due to Listeria monocytogenes in a Spanish Hospital over a Nine-Year Study Period, 2006–2014. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1054564
Modern Language Association (MLA)
Ariza-Miguel, Jaime…[et al.]. Molecular Epidemiology of Invasive Listeriosis due to Listeria monocytogenes in a Spanish Hospital over a Nine-Year Study Period, 2006–2014. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1054564
American Medical Association (AMA)
Ariza-Miguel, Jaime& Fernández-Natal, María Isabel& Soriano, Francisco& Hernández, Marta& Stessl, Beatrix& Rodriguez-Lazaro, D.. Molecular Epidemiology of Invasive Listeriosis due to Listeria monocytogenes in a Spanish Hospital over a Nine-Year Study Period, 2006–2014. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1054564
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054564