Hypoxia-Induced Epithelial-Mesenchymal Transition Is Involved in Bleomycin-Induced Lung Fibrosis
المؤلفون المشاركون
Guo, Liang
Xu, Jun-mei
Liu, Lei
Liu, Su-mei
Zhu, Rong
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-12-27
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Pulmonary fibrosis is a severe disease that contributes to the morbidity and mortality of a number of lung diseases.
However, the molecular and cellular mechanisms leading to lung fibrosis are poorly understood.
This study investigated the roles of epithelial-mesenchymal transition (EMT) and the associated molecular mechanisms in bleomycin-induced lung fibrosis.
The bleomycin-induced fibrosis animal model was established by intratracheal injection of a single dose of bleomycin.
Protein expression was measured by Western blot, immunohistochemistry, and immunofluorescence.
Typical lesions of lung fibrosis were observed 1 week after bleomycin injection.
A progressive increase in MMP-2, S100A4, α-SMA, HIF-1α, ZEB1, CD44, phospho-p44/42 (p-p44/42), and phospho-p38 MAPK (p-p38) protein levels as well as activation of EMT was observed in the lung tissues of bleomycin mice.
Hypoxia increased HIF-1α and ZEB1 expression and activated EMT in H358 cells.
Also, continuous incubation of cells under mild hypoxic conditions increased CD44, p-p44/42, and p-p38 protein levels in H358 cells, which correlated with the increase in S100A4 expression.
In conclusion, bleomycin induces progressive lung fibrosis, which may be associated with activation of EMT.
The fibrosis-induced hypoxia may further activate EMT in distal alveoli through a hypoxia-HIF-1α-ZEB1 pathway and promote the differentiation of lung epithelial cells into fibroblasts through phosphorylation of p38 MAPK and Erk1/2 proteins.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Guo, Liang& Xu, Jun-mei& Liu, Lei& Liu, Su-mei& Zhu, Rong. 2015. Hypoxia-Induced Epithelial-Mesenchymal Transition Is Involved in Bleomycin-Induced Lung Fibrosis. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1054683
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Guo, Liang…[et al.]. Hypoxia-Induced Epithelial-Mesenchymal Transition Is Involved in Bleomycin-Induced Lung Fibrosis. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1054683
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Guo, Liang& Xu, Jun-mei& Liu, Lei& Liu, Su-mei& Zhu, Rong. Hypoxia-Induced Epithelial-Mesenchymal Transition Is Involved in Bleomycin-Induced Lung Fibrosis. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1054683
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1054683
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر