Hypoxia-Induced Epithelial-Mesenchymal Transition Is Involved in Bleomycin-Induced Lung Fibrosis
Joint Authors
Guo, Liang
Xu, Jun-mei
Liu, Lei
Liu, Su-mei
Zhu, Rong
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-27
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Pulmonary fibrosis is a severe disease that contributes to the morbidity and mortality of a number of lung diseases.
However, the molecular and cellular mechanisms leading to lung fibrosis are poorly understood.
This study investigated the roles of epithelial-mesenchymal transition (EMT) and the associated molecular mechanisms in bleomycin-induced lung fibrosis.
The bleomycin-induced fibrosis animal model was established by intratracheal injection of a single dose of bleomycin.
Protein expression was measured by Western blot, immunohistochemistry, and immunofluorescence.
Typical lesions of lung fibrosis were observed 1 week after bleomycin injection.
A progressive increase in MMP-2, S100A4, α-SMA, HIF-1α, ZEB1, CD44, phospho-p44/42 (p-p44/42), and phospho-p38 MAPK (p-p38) protein levels as well as activation of EMT was observed in the lung tissues of bleomycin mice.
Hypoxia increased HIF-1α and ZEB1 expression and activated EMT in H358 cells.
Also, continuous incubation of cells under mild hypoxic conditions increased CD44, p-p44/42, and p-p38 protein levels in H358 cells, which correlated with the increase in S100A4 expression.
In conclusion, bleomycin induces progressive lung fibrosis, which may be associated with activation of EMT.
The fibrosis-induced hypoxia may further activate EMT in distal alveoli through a hypoxia-HIF-1α-ZEB1 pathway and promote the differentiation of lung epithelial cells into fibroblasts through phosphorylation of p38 MAPK and Erk1/2 proteins.
American Psychological Association (APA)
Guo, Liang& Xu, Jun-mei& Liu, Lei& Liu, Su-mei& Zhu, Rong. 2015. Hypoxia-Induced Epithelial-Mesenchymal Transition Is Involved in Bleomycin-Induced Lung Fibrosis. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1054683
Modern Language Association (MLA)
Guo, Liang…[et al.]. Hypoxia-Induced Epithelial-Mesenchymal Transition Is Involved in Bleomycin-Induced Lung Fibrosis. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1054683
American Medical Association (AMA)
Guo, Liang& Xu, Jun-mei& Liu, Lei& Liu, Su-mei& Zhu, Rong. Hypoxia-Induced Epithelial-Mesenchymal Transition Is Involved in Bleomycin-Induced Lung Fibrosis. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1054683
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054683