Differential Regulation of Proinflammatory Mediators following LPS- and ATP-Induced Activation of Monocytes from Patients with Antiphospholipid Syndrome
المؤلفون المشاركون
Navratilova, Zdenka
Manukyan, Gayane
Petrek, Martin
Martirosyan, Anush
Blbulyan, Armen
Boyajyan, Anna
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-02-15
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Antiphospholipid syndrome (APS) is an acquired autoimmune disorder characterized by recurrent thrombosis and pregnancy morbidity in association with the presence of antiphospholipid antibodies.
Growing evidence supports the involvement of monocytes in APS pathogenesis.
Inflammatory activation of monocytes promotes thrombus formation and other APS complications.
However, mechanisms underlying their activation are poorly investigated.
We aimed to determine transcriptional activity of monocytes after exposing them to low concentrations of lipopolysaccharide (LPS) and LPS + adenosine triphosphate (ATP) using comparative qRT-PCR.
The results showed that LPS significantly increased transcriptional levels of TLR2, IL-23, CCL2, CXCL10, IL-1β, and IL-6 in APS cells, while, in cells from healthy donors, LPS resulted in IL-6 and STAT3 elevated mRNAs.
Double stimulation of the cells resulted in decreased mRNA levels of NLRP3 in monocytes isolated from healthy donors and CCL2, IL-1β in APS cells.
By contrast, TLR2 mRNAs were elevated in both investigated groups after culture of the cells with LPS + ATP.
Thus, the findings indicate increased sensitivity of APS cells to LPS that may contribute to thrombus formation and enhance development or progression of autoimmune processes.
Low concentrations of ATP diminish LPS-induced inflammatory state of APS monocytes which might be a potential mechanism which regulates inflammatory state of the cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Martirosyan, Anush& Petrek, Martin& Navratilova, Zdenka& Blbulyan, Armen& Boyajyan, Anna& Manukyan, Gayane. 2015. Differential Regulation of Proinflammatory Mediators following LPS- and ATP-Induced Activation of Monocytes from Patients with Antiphospholipid Syndrome. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1054930
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Martirosyan, Anush…[et al.]. Differential Regulation of Proinflammatory Mediators following LPS- and ATP-Induced Activation of Monocytes from Patients with Antiphospholipid Syndrome. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1054930
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Martirosyan, Anush& Petrek, Martin& Navratilova, Zdenka& Blbulyan, Armen& Boyajyan, Anna& Manukyan, Gayane. Differential Regulation of Proinflammatory Mediators following LPS- and ATP-Induced Activation of Monocytes from Patients with Antiphospholipid Syndrome. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1054930
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1054930
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر