Differential Regulation of Proinflammatory Mediators following LPS- and ATP-Induced Activation of Monocytes from Patients with Antiphospholipid Syndrome
Joint Authors
Navratilova, Zdenka
Manukyan, Gayane
Petrek, Martin
Martirosyan, Anush
Blbulyan, Armen
Boyajyan, Anna
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-15
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Antiphospholipid syndrome (APS) is an acquired autoimmune disorder characterized by recurrent thrombosis and pregnancy morbidity in association with the presence of antiphospholipid antibodies.
Growing evidence supports the involvement of monocytes in APS pathogenesis.
Inflammatory activation of monocytes promotes thrombus formation and other APS complications.
However, mechanisms underlying their activation are poorly investigated.
We aimed to determine transcriptional activity of monocytes after exposing them to low concentrations of lipopolysaccharide (LPS) and LPS + adenosine triphosphate (ATP) using comparative qRT-PCR.
The results showed that LPS significantly increased transcriptional levels of TLR2, IL-23, CCL2, CXCL10, IL-1β, and IL-6 in APS cells, while, in cells from healthy donors, LPS resulted in IL-6 and STAT3 elevated mRNAs.
Double stimulation of the cells resulted in decreased mRNA levels of NLRP3 in monocytes isolated from healthy donors and CCL2, IL-1β in APS cells.
By contrast, TLR2 mRNAs were elevated in both investigated groups after culture of the cells with LPS + ATP.
Thus, the findings indicate increased sensitivity of APS cells to LPS that may contribute to thrombus formation and enhance development or progression of autoimmune processes.
Low concentrations of ATP diminish LPS-induced inflammatory state of APS monocytes which might be a potential mechanism which regulates inflammatory state of the cells.
American Psychological Association (APA)
Martirosyan, Anush& Petrek, Martin& Navratilova, Zdenka& Blbulyan, Armen& Boyajyan, Anna& Manukyan, Gayane. 2015. Differential Regulation of Proinflammatory Mediators following LPS- and ATP-Induced Activation of Monocytes from Patients with Antiphospholipid Syndrome. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1054930
Modern Language Association (MLA)
Martirosyan, Anush…[et al.]. Differential Regulation of Proinflammatory Mediators following LPS- and ATP-Induced Activation of Monocytes from Patients with Antiphospholipid Syndrome. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1054930
American Medical Association (AMA)
Martirosyan, Anush& Petrek, Martin& Navratilova, Zdenka& Blbulyan, Armen& Boyajyan, Anna& Manukyan, Gayane. Differential Regulation of Proinflammatory Mediators following LPS- and ATP-Induced Activation of Monocytes from Patients with Antiphospholipid Syndrome. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1054930
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054930