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Hypoxia Induces a Metabolic Shift and Enhances the Stemness and Expansion of Cochlear Spiral Ganglion StemProgenitor Cells
المؤلفون المشاركون
Sytwu, Huey-Kang
Chen, Hang-Kang
Lin, Yi-Chun
Shih, Cheng-Ping
Chen, Hsin-Chien
Kuo, Chao-Yin
Lee, Jen-Tin
Chao, Ting-Ting
Li, Shiue-Li
Fang, Mei-Cho
Wang, Chih-Hung
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-07-05
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Previously, we demonstrated that hypoxia (1% O2) enhances stemness markers and expands the cell numbers of cochlear stem/progenitor cells (SPCs).
In this study, we further investigated the long-term effect of hypoxia on stemness and the bioenergetic status of cochlear spiral ganglion SPCs cultured at low oxygen tensions.
Spiral ganglion SPCs were obtained from postnatal day 1 CBA/CaJ mouse pups.
The measurement of oxygen consumption rate, extracellular acidification rate (ECAR), and intracellular adenosine triphosphate levels corresponding to 20% and 5% oxygen concentrations was determined using a Seahorse XF extracellular flux analyzer.
After low oxygen tension cultivation for 21 days, the mean size of the hypoxia-expanded neurospheres was significantly increased at 5% O2; this correlated with high-level expression of hypoxia-inducible factor-1 alpha (Hif-1α), proliferating cell nuclear antigen (PCNA), cyclin D1, Abcg2, nestin, and Nanog proteins but downregulated expression of p27 compared to that in a normoxic condition.
Low oxygen tension cultivation tended to increase the side population fraction, with a significant difference found at 5% O2 compared to that at 20% O2.
In addition, hypoxia induced a metabolic energy shift of SPCs toward higher basal ECARs and higher maximum mitochondrial respiratory capacity but lower proton leak than under normoxia, where the SPC metabolism was switched toward glycolysis in long-term hypoxic cultivation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Hsin-Chien& Lee, Jen-Tin& Shih, Cheng-Ping& Chao, Ting-Ting& Sytwu, Huey-Kang& Li, Shiue-Li…[et al.]. 2015. Hypoxia Induces a Metabolic Shift and Enhances the Stemness and Expansion of Cochlear Spiral Ganglion StemProgenitor Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1055185
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Hsin-Chien…[et al.]. Hypoxia Induces a Metabolic Shift and Enhances the Stemness and Expansion of Cochlear Spiral Ganglion StemProgenitor Cells. BioMed Research International No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1055185
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Hsin-Chien& Lee, Jen-Tin& Shih, Cheng-Ping& Chao, Ting-Ting& Sytwu, Huey-Kang& Li, Shiue-Li…[et al.]. Hypoxia Induces a Metabolic Shift and Enhances the Stemness and Expansion of Cochlear Spiral Ganglion StemProgenitor Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1055185
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1055185
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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