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Hypoxia Induces a Metabolic Shift and Enhances the Stemness and Expansion of Cochlear Spiral Ganglion StemProgenitor Cells
Joint Authors
Sytwu, Huey-Kang
Chen, Hang-Kang
Lin, Yi-Chun
Shih, Cheng-Ping
Chen, Hsin-Chien
Kuo, Chao-Yin
Lee, Jen-Tin
Chao, Ting-Ting
Li, Shiue-Li
Fang, Mei-Cho
Wang, Chih-Hung
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-05
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Previously, we demonstrated that hypoxia (1% O2) enhances stemness markers and expands the cell numbers of cochlear stem/progenitor cells (SPCs).
In this study, we further investigated the long-term effect of hypoxia on stemness and the bioenergetic status of cochlear spiral ganglion SPCs cultured at low oxygen tensions.
Spiral ganglion SPCs were obtained from postnatal day 1 CBA/CaJ mouse pups.
The measurement of oxygen consumption rate, extracellular acidification rate (ECAR), and intracellular adenosine triphosphate levels corresponding to 20% and 5% oxygen concentrations was determined using a Seahorse XF extracellular flux analyzer.
After low oxygen tension cultivation for 21 days, the mean size of the hypoxia-expanded neurospheres was significantly increased at 5% O2; this correlated with high-level expression of hypoxia-inducible factor-1 alpha (Hif-1α), proliferating cell nuclear antigen (PCNA), cyclin D1, Abcg2, nestin, and Nanog proteins but downregulated expression of p27 compared to that in a normoxic condition.
Low oxygen tension cultivation tended to increase the side population fraction, with a significant difference found at 5% O2 compared to that at 20% O2.
In addition, hypoxia induced a metabolic energy shift of SPCs toward higher basal ECARs and higher maximum mitochondrial respiratory capacity but lower proton leak than under normoxia, where the SPC metabolism was switched toward glycolysis in long-term hypoxic cultivation.
American Psychological Association (APA)
Chen, Hsin-Chien& Lee, Jen-Tin& Shih, Cheng-Ping& Chao, Ting-Ting& Sytwu, Huey-Kang& Li, Shiue-Li…[et al.]. 2015. Hypoxia Induces a Metabolic Shift and Enhances the Stemness and Expansion of Cochlear Spiral Ganglion StemProgenitor Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1055185
Modern Language Association (MLA)
Chen, Hsin-Chien…[et al.]. Hypoxia Induces a Metabolic Shift and Enhances the Stemness and Expansion of Cochlear Spiral Ganglion StemProgenitor Cells. BioMed Research International No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1055185
American Medical Association (AMA)
Chen, Hsin-Chien& Lee, Jen-Tin& Shih, Cheng-Ping& Chao, Ting-Ting& Sytwu, Huey-Kang& Li, Shiue-Li…[et al.]. Hypoxia Induces a Metabolic Shift and Enhances the Stemness and Expansion of Cochlear Spiral Ganglion StemProgenitor Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1055185
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055185