3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study
المؤلفون المشاركون
Yu-Hua, Lu
Zhi-Wei, Wang
Wu, Di
Wan, Jian
Huang, Yan
Guo, Yibing
Xu, Tianxin
Zhu, Mingyan
Fan, Xiangjun
Ling, Changchun
Li, Xiaohong
Lu, Jingjing
Zhu, Hui
Zhou, Pengcheng
Sha-Jun, Zhu
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-11-24
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Type 1 diabetes is an autoimmune disease which is due to the lack of β cells.
The ideal therapy to cure the disease is pancreas transplantation, but its application is confined to a limited number of people due to the shortage of organ and the need for life-long immunosuppression.
Regenerative medicine methods such as a tissue engineered pancreas seem to provide a useful method.
In order to construct a microenvironment similar to the native pancreas that is suitable for not only cell growth but also cellular function exertion, a decellularized mouse pancreas was used as a natural 3D scaffold in this experiment.
MIN-6 β cells were planted in the bioscaffold.
The cell engraftment was verified by HE staining and SEM.
Immunostaining procedures were performed to confirm the normal function of the engrafted cells.
qRT-PCR demonstrated that insulin gene expression of the recellularized pancreas was upregulated compared with conventional plate-cultured cells.
In vivo experiment was also accomplished to further evaluate the function of the recellularized bioscaffold and the result was inspiring.
And beyond doubt this will bring new hope for type 1 diabetic patients.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wu, Di& Wan, Jian& Huang, Yan& Guo, Yibing& Xu, Tianxin& Zhu, Mingyan…[et al.]. 2015. 3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055472
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wu, Di…[et al.]. 3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1055472
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wu, Di& Wan, Jian& Huang, Yan& Guo, Yibing& Xu, Tianxin& Zhu, Mingyan…[et al.]. 3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055472
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1055472
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر