3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study
Joint Authors
Yu-Hua, Lu
Zhi-Wei, Wang
Wu, Di
Wan, Jian
Huang, Yan
Guo, Yibing
Xu, Tianxin
Zhu, Mingyan
Fan, Xiangjun
Ling, Changchun
Li, Xiaohong
Lu, Jingjing
Zhu, Hui
Zhou, Pengcheng
Sha-Jun, Zhu
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-24
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Type 1 diabetes is an autoimmune disease which is due to the lack of β cells.
The ideal therapy to cure the disease is pancreas transplantation, but its application is confined to a limited number of people due to the shortage of organ and the need for life-long immunosuppression.
Regenerative medicine methods such as a tissue engineered pancreas seem to provide a useful method.
In order to construct a microenvironment similar to the native pancreas that is suitable for not only cell growth but also cellular function exertion, a decellularized mouse pancreas was used as a natural 3D scaffold in this experiment.
MIN-6 β cells were planted in the bioscaffold.
The cell engraftment was verified by HE staining and SEM.
Immunostaining procedures were performed to confirm the normal function of the engrafted cells.
qRT-PCR demonstrated that insulin gene expression of the recellularized pancreas was upregulated compared with conventional plate-cultured cells.
In vivo experiment was also accomplished to further evaluate the function of the recellularized bioscaffold and the result was inspiring.
And beyond doubt this will bring new hope for type 1 diabetic patients.
American Psychological Association (APA)
Wu, Di& Wan, Jian& Huang, Yan& Guo, Yibing& Xu, Tianxin& Zhu, Mingyan…[et al.]. 2015. 3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055472
Modern Language Association (MLA)
Wu, Di…[et al.]. 3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1055472
American Medical Association (AMA)
Wu, Di& Wan, Jian& Huang, Yan& Guo, Yibing& Xu, Tianxin& Zhu, Mingyan…[et al.]. 3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055472
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055472