Identification of Novel Potential Vaccine Candidates against Tuberculosis Based on Reverse Vaccinology

المؤلفون المشاركون

Monterrubio-López, Gloria P.
Ribas-Aparicio, Rosa María
Gonzalez-y-Merchand, Jorge A.

المصدر

BioMed Research International

العدد

المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-16، 16ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2015-04-15

دولة النشر

مصر

عدد الصفحات

16

التخصصات الرئيسية

الطب البشري

الملخص EN

Tuberculosis (TB) is a chronic infectious disease, considered as the second leading cause of death worldwide, caused by Mycobacterium tuberculosis.

The limited efficacy of the bacillus Calmette-Guérin (BCG) vaccine against pulmonary TB and the emergence of multidrug-resistant TB warrants the need for more efficacious vaccines.

Reverse vaccinology uses the entire proteome of a pathogen to select the best vaccine antigens by in silico approaches.

M.

tuberculosis H37Rv proteome was analyzed with NERVE (New Enhanced Reverse Vaccinology Environment) prediction software to identify potential vaccine targets; these 331 proteins were further analyzed with VaxiJen for the determination of their antigenicity value.

Only candidates with values ≥0.5 of antigenicity and 50% of adhesin probability and without homology with human proteins or transmembrane regions were selected, resulting in 73 antigens.

These proteins were grouped by families in seven groups and analyzed by amino acid sequence alignments, selecting 16 representative proteins.

For each candidate, a search of the literature and protein analysis with different bioinformatics tools, as well as a simulation of the immune response, was conducted.

Finally, we selected six novel vaccine candidates, EsxL, PE26, PPE65, PE_PGRS49, PBP1, and Erp, from M.

tuberculosis that can be used to improve or design new TB vaccines.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Monterrubio-López, Gloria P.& Gonzalez-y-Merchand, Jorge A.& Ribas-Aparicio, Rosa María. 2015. Identification of Novel Potential Vaccine Candidates against Tuberculosis Based on Reverse Vaccinology. BioMed Research International،Vol. 2015, no. 2015, pp.1-16.
https://search.emarefa.net/detail/BIM-1055647

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Monterrubio-López, Gloria P.…[et al.]. Identification of Novel Potential Vaccine Candidates against Tuberculosis Based on Reverse Vaccinology. BioMed Research International No. 2015 (2015), pp.1-16.
https://search.emarefa.net/detail/BIM-1055647

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Monterrubio-López, Gloria P.& Gonzalez-y-Merchand, Jorge A.& Ribas-Aparicio, Rosa María. Identification of Novel Potential Vaccine Candidates against Tuberculosis Based on Reverse Vaccinology. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-16.
https://search.emarefa.net/detail/BIM-1055647

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1055647