Identification of Novel Potential Vaccine Candidates against Tuberculosis Based on Reverse Vaccinology
Joint Authors
Monterrubio-López, Gloria P.
Ribas-Aparicio, Rosa María
Gonzalez-y-Merchand, Jorge A.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-15
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Tuberculosis (TB) is a chronic infectious disease, considered as the second leading cause of death worldwide, caused by Mycobacterium tuberculosis.
The limited efficacy of the bacillus Calmette-Guérin (BCG) vaccine against pulmonary TB and the emergence of multidrug-resistant TB warrants the need for more efficacious vaccines.
Reverse vaccinology uses the entire proteome of a pathogen to select the best vaccine antigens by in silico approaches.
M.
tuberculosis H37Rv proteome was analyzed with NERVE (New Enhanced Reverse Vaccinology Environment) prediction software to identify potential vaccine targets; these 331 proteins were further analyzed with VaxiJen for the determination of their antigenicity value.
Only candidates with values ≥0.5 of antigenicity and 50% of adhesin probability and without homology with human proteins or transmembrane regions were selected, resulting in 73 antigens.
These proteins were grouped by families in seven groups and analyzed by amino acid sequence alignments, selecting 16 representative proteins.
For each candidate, a search of the literature and protein analysis with different bioinformatics tools, as well as a simulation of the immune response, was conducted.
Finally, we selected six novel vaccine candidates, EsxL, PE26, PPE65, PE_PGRS49, PBP1, and Erp, from M.
tuberculosis that can be used to improve or design new TB vaccines.
American Psychological Association (APA)
Monterrubio-López, Gloria P.& Gonzalez-y-Merchand, Jorge A.& Ribas-Aparicio, Rosa María. 2015. Identification of Novel Potential Vaccine Candidates against Tuberculosis Based on Reverse Vaccinology. BioMed Research International،Vol. 2015, no. 2015, pp.1-16.
https://search.emarefa.net/detail/BIM-1055647
Modern Language Association (MLA)
Monterrubio-López, Gloria P.…[et al.]. Identification of Novel Potential Vaccine Candidates against Tuberculosis Based on Reverse Vaccinology. BioMed Research International No. 2015 (2015), pp.1-16.
https://search.emarefa.net/detail/BIM-1055647
American Medical Association (AMA)
Monterrubio-López, Gloria P.& Gonzalez-y-Merchand, Jorge A.& Ribas-Aparicio, Rosa María. Identification of Novel Potential Vaccine Candidates against Tuberculosis Based on Reverse Vaccinology. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-16.
https://search.emarefa.net/detail/BIM-1055647
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055647