Antioxidant and Proapoptotic Activities of Sclerocarya birrea [(A. Rich.) Hochst.] Methanolic Root Extract on the Hepatocellular Carcinoma Cell Line HepG2
المؤلفون المشاركون
Bisaccia, Faustino
Armentano, Maria Francesca
Miglionico, Rocchina
Russo, Daniela
Nolfi, Nicoletta
Carmosino, Monica
Diop, Moussoukhoye Sissokho
Milella, Luigi
Valentao, Patricia
Andrade, Paula B.
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-05-17
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
The main goal of this study was to characterize the in vitro antioxidant activity and the apoptotic potential of S.
birrea methanolic root extract (MRE).
Among four tested extracts, obtained with different solvents, MRE showed the highest content of polyphenols, flavonoids, and tannins together with antioxidant activities tested with superoxide, nitric oxide, ABTS, and beta-carotene bleaching assays.
Moreover, the cytotoxic effect of MRE was evaluated on the hepatocarcinoma cell line HepG2.
In these cells, MRE treatment induced apoptosis and generated reactive oxygen species (ROS) in dose-dependent manner.
The cytotoxic effect promoted by MRE was prevented by pretreatment of HepG2 cells with N-acetyl-L-cysteine (NAC), suggesting that oxidative stress was pivotal in MRE-mediated cell death.
Moreover, we showed that the MRE treatment induced the mitochondrial membrane depolarization and the cytochrome c release from mitochondria into the cytosol.
It suggests that the apoptosis occurred in a mitochondrial-dependent pathway.
Interestingly, MRE showed a sensibly lower cytotoxicity, associated with a low increase of ROS, in normal human dermal fibroblasts compared to HepG2 cells.
It is suggested that the methanolic root extract of S.
Birrea is able to selectively increase intracellular ROS levels in cancer cells, promoting cell death.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Armentano, Maria Francesca& Bisaccia, Faustino& Miglionico, Rocchina& Russo, Daniela& Nolfi, Nicoletta& Carmosino, Monica…[et al.]. 2015. Antioxidant and Proapoptotic Activities of Sclerocarya birrea [(A. Rich.) Hochst.] Methanolic Root Extract on the Hepatocellular Carcinoma Cell Line HepG2. BioMed Research International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1055918
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Armentano, Maria Francesca…[et al.]. Antioxidant and Proapoptotic Activities of Sclerocarya birrea [(A. Rich.) Hochst.] Methanolic Root Extract on the Hepatocellular Carcinoma Cell Line HepG2. BioMed Research International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1055918
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Armentano, Maria Francesca& Bisaccia, Faustino& Miglionico, Rocchina& Russo, Daniela& Nolfi, Nicoletta& Carmosino, Monica…[et al.]. Antioxidant and Proapoptotic Activities of Sclerocarya birrea [(A. Rich.) Hochst.] Methanolic Root Extract on the Hepatocellular Carcinoma Cell Line HepG2. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1055918
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1055918
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر