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Antioxidant and Proapoptotic Activities of Sclerocarya birrea [(A. Rich.) Hochst.] Methanolic Root Extract on the Hepatocellular Carcinoma Cell Line HepG2
Joint Authors
Bisaccia, Faustino
Armentano, Maria Francesca
Miglionico, Rocchina
Russo, Daniela
Nolfi, Nicoletta
Carmosino, Monica
Diop, Moussoukhoye Sissokho
Milella, Luigi
Valentao, Patricia
Andrade, Paula B.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-17
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The main goal of this study was to characterize the in vitro antioxidant activity and the apoptotic potential of S.
birrea methanolic root extract (MRE).
Among four tested extracts, obtained with different solvents, MRE showed the highest content of polyphenols, flavonoids, and tannins together with antioxidant activities tested with superoxide, nitric oxide, ABTS, and beta-carotene bleaching assays.
Moreover, the cytotoxic effect of MRE was evaluated on the hepatocarcinoma cell line HepG2.
In these cells, MRE treatment induced apoptosis and generated reactive oxygen species (ROS) in dose-dependent manner.
The cytotoxic effect promoted by MRE was prevented by pretreatment of HepG2 cells with N-acetyl-L-cysteine (NAC), suggesting that oxidative stress was pivotal in MRE-mediated cell death.
Moreover, we showed that the MRE treatment induced the mitochondrial membrane depolarization and the cytochrome c release from mitochondria into the cytosol.
It suggests that the apoptosis occurred in a mitochondrial-dependent pathway.
Interestingly, MRE showed a sensibly lower cytotoxicity, associated with a low increase of ROS, in normal human dermal fibroblasts compared to HepG2 cells.
It is suggested that the methanolic root extract of S.
Birrea is able to selectively increase intracellular ROS levels in cancer cells, promoting cell death.
American Psychological Association (APA)
Armentano, Maria Francesca& Bisaccia, Faustino& Miglionico, Rocchina& Russo, Daniela& Nolfi, Nicoletta& Carmosino, Monica…[et al.]. 2015. Antioxidant and Proapoptotic Activities of Sclerocarya birrea [(A. Rich.) Hochst.] Methanolic Root Extract on the Hepatocellular Carcinoma Cell Line HepG2. BioMed Research International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1055918
Modern Language Association (MLA)
Armentano, Maria Francesca…[et al.]. Antioxidant and Proapoptotic Activities of Sclerocarya birrea [(A. Rich.) Hochst.] Methanolic Root Extract on the Hepatocellular Carcinoma Cell Line HepG2. BioMed Research International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1055918
American Medical Association (AMA)
Armentano, Maria Francesca& Bisaccia, Faustino& Miglionico, Rocchina& Russo, Daniela& Nolfi, Nicoletta& Carmosino, Monica…[et al.]. Antioxidant and Proapoptotic Activities of Sclerocarya birrea [(A. Rich.) Hochst.] Methanolic Root Extract on the Hepatocellular Carcinoma Cell Line HepG2. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1055918
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055918