Global Gene Expression Profiling and Alternative Splicing Events during the Chondrogenic Differentiation of Human Cartilage Endplate-Derived Stem Cells
المؤلفون المشاركون
Shang, Jin
Fan, Xin
Shangguan, Lei
Liu, Huan
Zhou, Yue
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-11-15
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Low back pain (LBP) is a very prevalent disease and degenerative disc diseases (DDDs) usually account for the LBP.
However, the pathogenesis of DDDs is complicated and difficult to elucidate.
Alternative splicing is a sophisticated regulatory process which greatly increases cellular complexity and phenotypic diversity of eukaryotic organisms.
In addition, the cartilage endplate-derived stem cells have been discovered and identified by our research group.
In this paper, we continue to investigate gene expression profiling and alternative splicing events during chondrogenic differentiation of cartilage endplate-derived stem cells.
We adopted Affymetrix Human Transcriptome Array 2.0 (HTA 2.0) to compare the transcriptional and splicing changes between the control and differentiated samples.
RT-PCR and quantitative PCR are used to validate the microarray results.
The GO and KEGG pathway analysis was also performed.
After bioinformatics analysis of the data, we detected 1953 differentially expressed genes.
In terms of alternative splicing, the Splicing Index algorithm was used to select alternatively spliced genes.
We detected 4411 alternatively spliced genes.
GO and KEGG pathway analysis also revealed several functionally involved biological processes and signaling pathways.
To our knowledge, this is the first study to investigate the alternative splicing mechanisms in chondrogenic differentiation of stem cells on a genome-wide scale.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Shang, Jin& Fan, Xin& Shangguan, Lei& Liu, Huan& Zhou, Yue. 2015. Global Gene Expression Profiling and Alternative Splicing Events during the Chondrogenic Differentiation of Human Cartilage Endplate-Derived Stem Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1056095
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Shang, Jin…[et al.]. Global Gene Expression Profiling and Alternative Splicing Events during the Chondrogenic Differentiation of Human Cartilage Endplate-Derived Stem Cells. BioMed Research International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1056095
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Shang, Jin& Fan, Xin& Shangguan, Lei& Liu, Huan& Zhou, Yue. Global Gene Expression Profiling and Alternative Splicing Events during the Chondrogenic Differentiation of Human Cartilage Endplate-Derived Stem Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1056095
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1056095
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر