Global Gene Expression Profiling and Alternative Splicing Events during the Chondrogenic Differentiation of Human Cartilage Endplate-Derived Stem Cells
Joint Authors
Shang, Jin
Fan, Xin
Shangguan, Lei
Liu, Huan
Zhou, Yue
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-15
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Low back pain (LBP) is a very prevalent disease and degenerative disc diseases (DDDs) usually account for the LBP.
However, the pathogenesis of DDDs is complicated and difficult to elucidate.
Alternative splicing is a sophisticated regulatory process which greatly increases cellular complexity and phenotypic diversity of eukaryotic organisms.
In addition, the cartilage endplate-derived stem cells have been discovered and identified by our research group.
In this paper, we continue to investigate gene expression profiling and alternative splicing events during chondrogenic differentiation of cartilage endplate-derived stem cells.
We adopted Affymetrix Human Transcriptome Array 2.0 (HTA 2.0) to compare the transcriptional and splicing changes between the control and differentiated samples.
RT-PCR and quantitative PCR are used to validate the microarray results.
The GO and KEGG pathway analysis was also performed.
After bioinformatics analysis of the data, we detected 1953 differentially expressed genes.
In terms of alternative splicing, the Splicing Index algorithm was used to select alternatively spliced genes.
We detected 4411 alternatively spliced genes.
GO and KEGG pathway analysis also revealed several functionally involved biological processes and signaling pathways.
To our knowledge, this is the first study to investigate the alternative splicing mechanisms in chondrogenic differentiation of stem cells on a genome-wide scale.
American Psychological Association (APA)
Shang, Jin& Fan, Xin& Shangguan, Lei& Liu, Huan& Zhou, Yue. 2015. Global Gene Expression Profiling and Alternative Splicing Events during the Chondrogenic Differentiation of Human Cartilage Endplate-Derived Stem Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1056095
Modern Language Association (MLA)
Shang, Jin…[et al.]. Global Gene Expression Profiling and Alternative Splicing Events during the Chondrogenic Differentiation of Human Cartilage Endplate-Derived Stem Cells. BioMed Research International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1056095
American Medical Association (AMA)
Shang, Jin& Fan, Xin& Shangguan, Lei& Liu, Huan& Zhou, Yue. Global Gene Expression Profiling and Alternative Splicing Events during the Chondrogenic Differentiation of Human Cartilage Endplate-Derived Stem Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1056095
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056095