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Optimization of Culture Conditions for Production of the Anti-Leukemic Glutaminase Free L-Asparaginase by Newly Isolated Streptomyces olivaceus NEAE-119 Using Response Surface Methodology
المؤلفون المشاركون
El-Naggar, Noura El-Ahmady
Moawad, Hassan
El-Shweihy, Nancy M.
El-Ewasy, Sara M.
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-06-09
دولة النشر
مصر
عدد الصفحات
17
التخصصات الرئيسية
الملخص EN
Among the antitumor drugs, bacterial enzyme L-asparaginase has been employed as the most effective chemotherapeutic agent in pediatric oncotherapy especially for acute lymphoblastic leukemia.
Glutaminase free L-asparaginase producing actinomycetes were isolated from soil samples collected from Egypt.
Among them, a potential culture, strain NEAE-119, was selected and identified on the basis of morphological, cultural, physiological, and biochemical properties together with 16S rRNA sequence as Streptomyces olivaceus NEAE-119 and sequencing product (1509 bp) was deposited in the GenBank database under accession number KJ200342.
The optimization of different process parameters for L-asparaginase production by Streptomyces olivaceus NEAE-119 using Plackett-Burman experimental design and response surface methodology was carried out.
Fifteen variables (temperature, pH, incubation time, inoculum size, inoculum age, agitation speed, dextrose, starch, L-asparagine, KNO3, yeast extract, K2HPO4, MgSO4 · 7H2O, NaCl, and FeSO4 · 7H2O) were screened using Plackett-Burman experimental design.
The most positive significant independent variables affecting enzyme production (temperature, inoculum age, and agitation speed) were further optimized by the face-centered central composite design-response surface methodology.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
El-Naggar, Noura El-Ahmady& Moawad, Hassan& El-Shweihy, Nancy M.& El-Ewasy, Sara M.. 2015. Optimization of Culture Conditions for Production of the Anti-Leukemic Glutaminase Free L-Asparaginase by Newly Isolated Streptomyces olivaceus NEAE-119 Using Response Surface Methodology. BioMed Research International،Vol. 2015, no. 2015, pp.1-17.
https://search.emarefa.net/detail/BIM-1056188
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
El-Naggar, Noura El-Ahmady…[et al.]. Optimization of Culture Conditions for Production of the Anti-Leukemic Glutaminase Free L-Asparaginase by Newly Isolated Streptomyces olivaceus NEAE-119 Using Response Surface Methodology. BioMed Research International No. 2015 (2015), pp.1-17.
https://search.emarefa.net/detail/BIM-1056188
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
El-Naggar, Noura El-Ahmady& Moawad, Hassan& El-Shweihy, Nancy M.& El-Ewasy, Sara M.. Optimization of Culture Conditions for Production of the Anti-Leukemic Glutaminase Free L-Asparaginase by Newly Isolated Streptomyces olivaceus NEAE-119 Using Response Surface Methodology. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-17.
https://search.emarefa.net/detail/BIM-1056188
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1056188
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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