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Optimization of Culture Conditions for Production of the Anti-Leukemic Glutaminase Free L-Asparaginase by Newly Isolated Streptomyces olivaceus NEAE-119 Using Response Surface Methodology
Joint Authors
El-Naggar, Noura El-Ahmady
Moawad, Hassan
El-Shweihy, Nancy M.
El-Ewasy, Sara M.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-09
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Among the antitumor drugs, bacterial enzyme L-asparaginase has been employed as the most effective chemotherapeutic agent in pediatric oncotherapy especially for acute lymphoblastic leukemia.
Glutaminase free L-asparaginase producing actinomycetes were isolated from soil samples collected from Egypt.
Among them, a potential culture, strain NEAE-119, was selected and identified on the basis of morphological, cultural, physiological, and biochemical properties together with 16S rRNA sequence as Streptomyces olivaceus NEAE-119 and sequencing product (1509 bp) was deposited in the GenBank database under accession number KJ200342.
The optimization of different process parameters for L-asparaginase production by Streptomyces olivaceus NEAE-119 using Plackett-Burman experimental design and response surface methodology was carried out.
Fifteen variables (temperature, pH, incubation time, inoculum size, inoculum age, agitation speed, dextrose, starch, L-asparagine, KNO3, yeast extract, K2HPO4, MgSO4 · 7H2O, NaCl, and FeSO4 · 7H2O) were screened using Plackett-Burman experimental design.
The most positive significant independent variables affecting enzyme production (temperature, inoculum age, and agitation speed) were further optimized by the face-centered central composite design-response surface methodology.
American Psychological Association (APA)
El-Naggar, Noura El-Ahmady& Moawad, Hassan& El-Shweihy, Nancy M.& El-Ewasy, Sara M.. 2015. Optimization of Culture Conditions for Production of the Anti-Leukemic Glutaminase Free L-Asparaginase by Newly Isolated Streptomyces olivaceus NEAE-119 Using Response Surface Methodology. BioMed Research International،Vol. 2015, no. 2015, pp.1-17.
https://search.emarefa.net/detail/BIM-1056188
Modern Language Association (MLA)
El-Naggar, Noura El-Ahmady…[et al.]. Optimization of Culture Conditions for Production of the Anti-Leukemic Glutaminase Free L-Asparaginase by Newly Isolated Streptomyces olivaceus NEAE-119 Using Response Surface Methodology. BioMed Research International No. 2015 (2015), pp.1-17.
https://search.emarefa.net/detail/BIM-1056188
American Medical Association (AMA)
El-Naggar, Noura El-Ahmady& Moawad, Hassan& El-Shweihy, Nancy M.& El-Ewasy, Sara M.. Optimization of Culture Conditions for Production of the Anti-Leukemic Glutaminase Free L-Asparaginase by Newly Isolated Streptomyces olivaceus NEAE-119 Using Response Surface Methodology. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-17.
https://search.emarefa.net/detail/BIM-1056188
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056188