A High-Performance Multiplex Immunoassay for Serodiagnosis of Flavivirus-Associated Neurological Diseases in Horses
المؤلفون المشاركون
Lecollinet, Sylvie
Beck, Cécile
Desprès, Philippe
Paulous, Sylvie
Vanhomwegen, Jessica
Lowenski, Steeve
Nowotny, Norbert
Durand, Benoit
Garnier, Annabelle
Blaise-Boisseau, Sandra
Guitton, Edouard
Yamanaka, Takashi
Zientara, Stéphan
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-09-17
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
West Nile virus (WNV), Japanese encephalitis virus (JEV), and tick-borne encephalitis virus (TBEV) are flaviviruses responsible for severe neuroinvasive infections in humans and horses.
The confirmation of flavivirus infections is mostly based on rapid serological tests such as enzyme-linked immunosorbent assays (ELISAs).
These tests suffer from poor specificity, mainly due to antigenic cross-reactivity among flavivirus members.
Robust diagnosis therefore needs to be validated through virus neutralisation tests (VNTs) which are time-consuming and require BSL3 facilities.
The flavivirus envelope (E) glycoprotein ectodomain is composed of three domains (D) named DI, DII, and DIII, with EDIII containing virus-specific epitopes.
In order to improve the serological differentiation of flavivirus infections, the recombinant soluble ectodomain of WNV E (WNV.sE) and EDIIIs (rEDIIIs) of WNV, JEV, and TBEV were synthesised using the Drosophila S2 expression system.
Purified antigens were covalently bonded to fluorescent beads.
The microspheres coupled to WNV.sE or rEDIIIs were assayed with about 300 equine immune sera from natural and experimental flavivirus infections and 172 nonimmune equine sera as negative controls.
rEDIII-coupled microspheres captured specific antibodies against WNV, TBEV, or JEV in positive horse sera.
This innovative multiplex immunoassay is a powerful alternative to ELISAs and VNTs for veterinary diagnosis of flavivirus-related diseases.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Beck, Cécile& Desprès, Philippe& Paulous, Sylvie& Vanhomwegen, Jessica& Lowenski, Steeve& Nowotny, Norbert…[et al.]. 2015. A High-Performance Multiplex Immunoassay for Serodiagnosis of Flavivirus-Associated Neurological Diseases in Horses. BioMed Research International،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1056353
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Beck, Cécile…[et al.]. A High-Performance Multiplex Immunoassay for Serodiagnosis of Flavivirus-Associated Neurological Diseases in Horses. BioMed Research International No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1056353
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Beck, Cécile& Desprès, Philippe& Paulous, Sylvie& Vanhomwegen, Jessica& Lowenski, Steeve& Nowotny, Norbert…[et al.]. A High-Performance Multiplex Immunoassay for Serodiagnosis of Flavivirus-Associated Neurological Diseases in Horses. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1056353
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1056353
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر